Methods
Year tested: //1954 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1956 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1957 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1958 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1959 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1960 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1961 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1962 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1963 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1964 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1965 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1966 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1967 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1968 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1969 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1970 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1971 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1972 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1973 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1974 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1975 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1976 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1977 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1978 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1981 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1984 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Year tested: //1985 Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene (Stephanie.Greene@ars.usda.gov)
Kura clover general evaluation Experiment Type: Field Field Study Year: 1990 Exp. Design: RCB Year started: //1988 Year ended: //1990 Comment: Agronomic evaluation determined on spaced plants in a 4 replicate RCB design experiment.
Seeds of each plant introduction line were initiated in the greenhouse and transplanted into the field after approximately 4 weeks of growth. The field nursery consisted of four replications with each replication composed of seven plant rows for a total of 28 plants evaluated per plant introduction line. Data were collected for each charater based on the descriptors as listed in GRIN. Data were collected on a individual plant basis for crown width, growth habit, plant type, vigor, regowth, spring plant size, flower color, leaf mark, leaf color, leaf size, leafiness, stand survival, and plant height. Data for uniformity, maturity, flower production, and stolon density were recorded on a plot basis (seven plant row plots). Measured characters were collected in centimeters (plant height), percentage of plants (stand survival), or number of stolons intersecting a meter length (stolon density).
Study Name: Agronomic evaluation of Trifolium species. Experiment Type: Field field Study Year: 1989 Exp. Design: RCBD Exp. Location: Lexington Comment: Agronomic evaluation of 48 Trifolium accessions for vigor, growth habit, leaf mark, flower color, seeds per head.
Study Name: Characterization of agronomic traits in T. medium Experiment Type: Field field Study Year: 1990 Exp. Design: RCBD Exp. Location: Lexington Year started: 08/01/1989 Year seeded: 08/01/1989 Comment: Agronomic evaluation of Trifolium medium for vigor, spread leaf\ Agronomic evaluation of Trifolium medium for vigor, spread, leaf mark, flower color, leaf size, and flowering.
Study Name: T. repens agronomic study Experiment Type: Field field Study Year: 1991 Comment: Plant material evaluated for agronomic traits during the 1991 growing season.
Seventeen plants of each accession were grown in pots filled with Promix inthe reenhouse at temperature of about 18C. The youngest fully developed leafand petiole from each plant was homogenizied by hand in a 1.5ul centrifugetube containing 90ul of extraction buffer at -20C. The homogenized extractswas centrifuged at 8160 x g for five minutes and either used immediately orstored at -20Cfor a few hours. Isoelectric focusing was performed on agarose gels. Sevenenzyme systems, namely, diaphorase (DIA, E.C. 1.8.1.4), esterase (EST, E.C.3.1.1.-), glucose-6-phosphate isomerase (GPI, E.C. 5.3.1.9),beta-glucosidase (GLU, E.C. 3.2.1.21), peroxidase (PRX, E.C. 1.11.1.7),phosphoglucomutase (PGM, E.C. 2.7.5.1), and superoxide dismutase (SOD, E.C.1.15.1.1), resolved 10 isozyme loci with 28 alleles. Standard stainingrecipes were followed(Acquaah 1992; Wendel and Weeden 1989).
Seventeen plants of each accession were grown in pots filled with Promix inthe reenhouse at temperature of about 18C. The youngest fully developed leafand petiole from each plant was homogenizied by hand in a 1.5ul centrifugetube containing 90ul of extraction buffer at -20C. The homogenized extractswas centrifuged at 8160 x g for five minutes and either used immediately orstored at -20Cfor a few hours. Isoelectric focusing was performed on agarose gels. Sevenenzyme systems, namely, diaphorase (DIA, E.C. 1.8.1.4), esterase (EST, E.C.3.1.1.-), glucose-6-phosphate isomerase (GPI, E.C. 5.3.1.9),beta-glucosidase (GLU, E.C. 3.2.1.21), peroxidase (PRX, E.C. 1.11.1.7),phosphoglucomutase (PGM, E.C. 2.7.5.1), and superoxide dismutase (SOD, E.C.1.15.1.1), resolved 10 isozyme loci with 28 alleles. Standard stainingrecipes were followed(Acquaah 1992; Wendel and Weeden 1989).
Isoflavone content of Biochanina A on Red Clover(Trifolium pratense) by D.S. Wofford & Ken Quesenberry, Gainsville, Florida. 1: Sampled 2 to 4 g fully expanded leaves from each plant. Plants were 9.5 weeks age from time of transplanting into 150 cc conetainers. Plants were grown in a greenhopuse in Gainsville, FL during the period of October 15 to December 21, 1997. 2: Weigh a 1 to 2-gram subsample(record exact weight) of leaves and place into extraction vial and cover with 15ml of HPLC-grade methanol. 3: Weigh the remaining leaves for fresh weight, dry the sample and determine percent dry matter. 4: Place samples into refrigerator for 7 days at 7C. After 7 days remove vials and wash with 10ml HPLC-grade methanol so final sample is 25ml.
Study Name: Chromosome counts of Trifolium species. Experiment Type: Laboratory laboratory Study Year: 1988 Comment: Chromosome counts determined on T. medium, T. canescens, T. sarosiense, T. repens, T. longidentatum, T. pratense, T. stoloniferum
Chromosome counts done by R.R. Smith, Madison Wisconsin 1995 on Trifolium ambiguum.
Study Name: Core collection as determined by the Clover CAC. Comment: This study identifies accessions which comprise the core collection for red clover (Trifolium pratense) as determined by the Clover and Special Purpose Forage Legume Crop Advisory Committee (CAC).
Study Name: Core collection as determined by the Clover CAC. Comment: This study identifies accessions which comprise the core collection for white clover (Trifolium repens) as determined by the Clover and Special Purpose Forage Legume Crop Advisory Committee (CAC).
Contains data taken at the Southern Regional PI Station. The data in this study was recorded by the staff of the Southern Regional Plant Introduction Station in Griffin, GA.
Isoflavone content of Formononet on Red Clover(Trifolium pratense) by D.S. Wofford & Ken Quesenberry, Gainsville, Florida. 1: Sampled 2 to 4 g fully expanded leaves from each plant. Plants were 9.5 weeks age from time of transplanting into 150 cc conetainers. Plants were grown in a greenhopuse in Gainsville, FL during the period of October 15 to December 21, 1997. 2: Weigh a 1 to 2-gram subsample(record exact weight) of leaves and place into extraction vial and cover with 15ml of HPLC-grade methanol. 3: Weigh the remaining leaves for fresh weight, dry the sample and determine percent dry matter. 4: Place samples into refrigerator for 7 days at 7C. After 7 days remove vials and wash with 10ml HPLC-grade methanol so final sample is 25ml.
Isoflavone content of Genistein on Red Clover(Trifolium pratense) by D.S. Wofford & Ken Quesenberry, Gainsville, Florida. 1: Sampled 2 to 4 g fully expanded leaves from each plant. Plants were 9.5 weeks age from time of transplanting into 150 cc conetainers. Plants were grown in a greenhopuse in Gainsville, FL during the period of October 15 to December 21, 1997. 2: Weigh a 1 to 2-gram subsample(record exact weight) of leaves and place into extraction vial and cover with 15ml of HPLC-grade methanol. 3: Weigh the remaining leaves from fresh weight; dry the sampl and determine percent dry matter. 4: Place sample into refrigerator for 7 days at 7 C. After 7 days remove vials and wash with 10ml HPLC-grade methanol so final sample is 25ml. Units measured was mg per gram, which is equivalent to g/kg (GRIN units used)
Study Name: Cyanogenesis in White Clover Experiment Type: Greenhouse greenhouse Study Year: 1992 Year tested: 01/01/1992 Comment: Cyanogenesis was determined by the picric acid test (J.Amer. Soc. Agron. 34: 199-200) on 2-3 leaves on each of 20 plants grown in the greenhouse at Mississippi State, MS in 1992.
Seventeen plants of each accession were grown in pots filled with Promix inthe reenhouse at temperature of about 18C. The youngest fully developed leafand petiole from each plant was homogenizied by hand in a 1.5ul centrifugetube containing 90ul of extraction buffer at -20C. The homogenized extractswas centrifuged at 8160 x g for five minutes and either used immediately orstored at -20Cfor a few hours. Isoelectric focusing was performed on agarose gels. Sevenenzyme systems, namely, diaphorase (DIA, E.C. 1.8.1.4), esterase (EST, E.C.3.1.1.-), glucose-6-phosphate isomerase (GPI, E.C. 5.3.1.9),beta-glucosidase (GLU, E.C. 3.2.1.21), peroxidase (PRX, E.C. 1.11.1.7),phosphoglucomutase (PGM, E.C. 2.7.5.1), and superoxide dismutase (SOD, E.C.1.15.1.1), resolved 10 isozyme loci with 28 alleles. Standard stainingrecipes were followed(Acquaah 1992; Wendel and Weeden 1989).
Seventeen plants of each accession were grown in pots filled with Promix inthe reenhouse at temperature of about 18C. The youngest fully developed leafand petiole from each plant was homogenizied by hand in a 1.5ul centrifugetube containing 90ul of extraction buffer at -20C. The homogenized extractswas centrifuged at 8160 x g for five minutes and either used immediately orstored at -20Cfor a few hours. Isoelectric focusing was performed on agarose gels. Sevenenzyme systems, namely, diaphorase (DIA, E.C. 1.8.1.4), esterase (EST, E.C.3.1.1.-), glucose-6-phosphate isomerase (GPI, E.C. 5.3.1.9),beta-glucosidase (GLU, E.C. 3.2.1.21), peroxidase (PRX, E.C. 1.11.1.7),phosphoglucomutase (PGM, E.C. 2.7.5.1), and superoxide dismutase (SOD, E.C.1.15.1.1), resolved 10 isozyme loci with 28 alleles. Standard stainingrecipes were followed(Acquaah 1992; Wendel and Weeden 1989).
Study Name: In vitro regenerative capacity of trifolium species. Experiment Type: Laboratory laboratory Study Year: 1990 Comment: Percent of pre-embryogenic nodular callus produced by Trifolium pratense and related species.
Study Name: White clover resistance to M. graminicola Experiment Type: Greenhouse greenhouse Study Year: 1993 Comment: 261 accessions of white clover were evaluated for resistance to Meloidogyne graminicola.
Study Name: Meloidogyne resistance Experiment Type: Greenhouse greenhouse Study Year: 1993 Year started: 01/01/1993 Year ended: 10/01/1993 Comment: Gall and egg rating of M. arenaria, M. hapla, M. incognita, M. javanica taken on greenhouse grown plant material.
Study Name: Powdery Mildew
Red Clover (Trifolium pratense) Core collection evaluated for reaction to Mycoleptodiscus caused be "Mycoleptodiscus terrestris". Evaluation was done by Dr. Richard R. Smith, Madison, Wisconsin from Dec. 1998 to March 1999 in a growth chamber. Procedures used were developed by R.R. Smith and C.R. Craig.
Comment: The data in this study was recorded by the staff of the Plant Genetic Resources Unit in Geneva, New York. For additional information, contact Stephanie Greene at (315) 787-2292.
Study Name: Nematode resistance study Experiment Type: Greenhouse greenhouse Study Year: 1992 Comment: Clovers were evaluated for response to root knot nematode in 1992.
Seedling plants transplanted to 150 cm3. Conetainers filled with fine sand topsoil. Incolated with 10 egg per cm3(1500 per cone) of Meloidogyne arenaria race 1 at three weeks after tranplanting. Uprooted at 6-8 weeks after inoculation. Root systems evaluated for galls and egg masses. LITERATURE CITATIONS: Quesenberry, K.H., D.D. Baltensperger and R.A. Dunn. 1986. Screening Trifolium spp. for response to Meloidogyne spp. Crop Sci. 26:61-64.
Year tested: //1991 Comment: Data on resistance to 4 species of nematodes (arenaria, hapla, incognita, and javanica) in both the gall and egg stages was recorded on perennial clovers.
Red Clover (Trifolium pratense) Core collection evaluated for reaction to Northern Anthracnose caused by Auerobasidium caulivora.. Evaluation was done by Dr. Richard R. Smith, Madison, Wisconsin from Dec. 1998 to March 1999 in a growth chamber. Procedures used were developed by R.R. Smith and D.P. Maxwell. See: Anthracnose resistance in Red Clover. Crop Sci.13:271-274. Disease severity index used:1 = no visable symptoms, 2 = slight necrosis of the leaflet or petiole, 3 = necrosis of the petiole extending into leaflet, 4 = necrosis of the leaflets, 5 = extensive necrosis of the leaflets and lesions on the petiole.
Seventeen plants of each accession were grown in pots filled with Promix inthe reenhouse at temperature of about 18C. The youngest fully developed leafand petiole from each plant was homogenizied by hand in a 1.5ul centrifugetube containing 90ul of extraction buffer at -20C. The homogenized extractswas centrifuged at 8160 x g for five minutes and either used immediately orstored at -20Cfor a few hours. Isoelectric focusing was performed on agarose gels. Sevenenzyme systems, namely, diaphorase (DIA, E.C. 1.8.1.4), esterase (EST, E.C.3.1.1.-), glucose-6-phosphate isomerase (GPI, E.C. 5.3.1.9),beta-glucosidase (GLU, E.C. 3.2.1.21), peroxidase (PRX, E.C. 1.11.1.7),phosphoglucomutase (PGM, E.C. 2.7.5.1), and superoxide dismutase (SOD, E.C.1.15.1.1), resolved 10 isozyme loci with 28 alleles. Standard stainingrecipes were followed(Acquaah 1992; Wendel and Weeden 1989).
Study Name: Red clover Aphanomyces evaluation Experiment Type: Growth chamber Growth chamber Comment: Evaluation of red clover for resistance to Aphanomyces euteiches.
Study Name: Red clover Stemphylium evaluation Comment: Evaluation of red clover for resistance to Stemphylium sarcineforme.
Study Name: Resistance to Clyindrocladium in clover Experiment Type: Greenhouse Greenhouse Comment: A total of 150 seeds were used for each entry with half receiving inoculum and the other half serving as the uninoculated control for that entry.
Study Name: Resistance to Clyindrocladium in clover Experiment Type: Greenhouse Greenhouse Year tested: //1991 Comment: Percent resistance to Cylindrocladium crotalariae based on percent of an uninoculated control group for each entry. A total of 150 seed were used for each entry with half receiving inoculum and the other half serving as the uninoculated control for that entry.
100 seed weight at the Western Regional Plant Introduction Station, Pullman Washington, USA
Study Name: White clover general evaluation Experiment Type: Field Field Study Year: 1990 Exp. Design: RCB Year started: //1989 Year ended: //1990 Comment: Crown width, plant size, leafiness and growth habit determined on spaced plants in 10 replicate RCB design in May 1990. Other descriptors determined on 10-plant rows in 4 replicate RCB design in April and August 1990.
Study Name: Cyanogenesis of white clover Study Year: 1993 Comment: Cyanogenesis was determined by the picric acid test (J. Amer. Soc. Agronomy 39:199-200) on 2-3 leaves on each of 20 plants grown in the greenhouse in January, 1993.
Collection pictures from accessions collected in the United States
Collection pictures taken in 2000 from the Kazakstan trip made by Rich Hannan, Stephanie Greene, A. Khusainov, A. Afonin, and N. Dzyubenko.
Collection pictures taken in 2006 from the Tajikistan trip by Barbara Hellier, Kenneth Street, Zebuniso Muminshoeva, Farkhod Kosimov, Shakhlo Safarzoda, John Sheppard, Natalya Rukhkyan and Sergey Shuvalov.
Tajikistan collection trip map.
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington
Images/pictures from Prosser, Washington