Evaluation location: Florida, United States
Isoflavone content of Genistein on Red Clover(Trifolium pratense) by D.S. Wofford & Ken Quesenberry, Gainsville, Florida. 1: Sampled 2 to 4 g fully expanded leaves from each plant. Plants were 9.5 weeks age from time of transplanting into 150 cc conetainers. Plants were grown in a greenhopuse in Gainsville, FL during the period of October 15 to December 21, 1997. 2: Weigh a 1 to 2-gram subsample(record exact weight) of leaves and place into extraction vial and cover with 15ml of HPLC-grade methanol. 3: Weigh the remaining leaves from fresh weight; dry the sampl and determine percent dry matter. 4: Place sample into refrigerator for 7 days at 7 C. After 7 days remove vials and wash with 10ml HPLC-grade methanol so final sample is 25ml. Units measured was mg per gram, which is equivalent to g/kg (GRIN units used)