Evaluation location: Florida, United States
Isoflavone content of Biochanina A on Red Clover(Trifolium pratense) by D.S. Wofford & Ken Quesenberry, Gainsville, Florida. 1: Sampled 2 to 4 g fully expanded leaves from each plant. Plants were 9.5 weeks age from time of transplanting into 150 cc conetainers. Plants were grown in a greenhopuse in Gainsville, FL during the period of October 15 to December 21, 1997. 2: Weigh a 1 to 2-gram subsample(record exact weight) of leaves and place into extraction vial and cover with 15ml of HPLC-grade methanol. 3: Weigh the remaining leaves for fresh weight, dry the sample and determine percent dry matter. 4: Place samples into refrigerator for 7 days at 7C. After 7 days remove vials and wash with 10ml HPLC-grade methanol so final sample is 25ml.