Methods
Collection pictures from the Richard C. Johnson, Harold Bockelman, Korzhenevsky, V., Boguslavsky, R. collection in 1999 from Krym, Ukraine.
Pictures taken in 2005 from field growing plants at Pullman, Washington
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Days to first flower data taken at Karaj Agricultural College, Karaj, Iran. Nursery was planted March 30, 1965.
Pods per peduncle, Plant height at physiological maturity, Height of lowest peduncle, Seed production (yield) calcuated in kilograms per hectare. Data came from Spillman Farm near Pullman Washington in 1980. On May 18th, 1980 Spillman Farm received .5-2 cm of Mt. St Helens ash.
Excel table with the 1980 data.
Lentil accessions were planted on Arpil 26, 2001 (day 116 of the year) at Whitlow farm near Pullman, Washington. Plot was for regeneration/increase of the accessions, thus there was one rep planted of 300 seeds.
For an Excel file with the 2001 Lens data click here.
Accessions were planted on April 30, 2007 (day 120 of the year) at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
Aerial photo of Whitlow farm in Pullman, Wa of the 2007 nursery.
Pullman weather data from September 1, 2006 to August 31, 2007 as an Excel file .
Accessions were planted in April, 2008 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
The Palmer, Alaska (61.60 latitude 149.08 longitude, 113 meters altitude) growing season is very short (approximately 90-100 days) with up to 20 hours of continuous daylight. Planting took place the first week of June in cool, damp silty loam soils. The 2009 growing season consisted of mostly warm, dry days with an average high temperature of 75.3?F and average day length of 18 hours. There was very little rain and supplemental overhead irrigation was used when necessary. The major impediment during the season was a healthy weed population consisting mostly of Lambsquarters and chickweed. All weed control was done by hand.
For a Excel file of the 2009 Palmer Alaska data.
Accessions were planted in April, 2009 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
Accessions were planted in April, 2009 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions planted in 2010 in the greenhouses at Pullman, Washington
Accessions were planted in April, 2012 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted in April, 2013 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted in April, 2014 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted in April 8, 2016 day 98 of the year) at the Plant Materials farm (Field A) near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
Accessions were planted May 13, 2016 (day 133 of the year) at Spillman Farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted April 25, 2017 (day 115 of the year) at Spillman Farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted April 25, 2017 (day 115 of the year) at Spillman Farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds. Seeds planted came from single plant selection accessions. (LSP = Lens single plant)
Accessions were planted March 29, 2018 (day 88 of the year) at Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Accessions were planted on April, 2008 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
For a Excel file of the 2008 data
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Images of Lens taken in 2011 on the single plant selected accessions
Accessions were planted on April, 2012 at Whitlow farm near Pullman, Washington. Elevation 2500 feet, Latitude 46 degrees, 43 minutes, 55 seconds, Longitude 117 degrees, 9 minutes, 25 seconds.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
These seeds will be planted for increase in 2014 in Pullman, Washington. These accessions come from a single plant selection.
Scanner was an Epson Expression 10000XL flatbed scanner. Software is WinSEEDLE V. 2007c software with calibrated color correction, and set up the scans using the Regent Positioning System. The seed images are scanned at 400 dpi and saved in the .tif format.
For color comparison, the Munsell ColorChecker Chart from Xrite.com is included in the seed scan screen. The Munsell color system is a color space that specifies colors based on three color dimensions, hue, value ( lightness), and chroma (color purity or colorfulness). It was created by Professor Albert H. Munsell in the first decade of the 20th century. Reference: Munsell, Albert H. (January 1912). "A Pigment Color System and Notation." The American Journal of Psychology 23: 236-244.
These seeds will be planted for increase in 2014 in Pullman, Washington. These accessions come from a single plant selection.
Plants (Lentil ? Single Plant Core) were grown in 5-liter black plastic pots filled with a 2:1 (vol:vol) mixture of synthetic soil (Metro-Mix 360; Scotts-Sierra Horticultural Products Co., Marysville, Ohio, USA) and vermiculite (Strong-Lite Medium Vermiculite, Sun Gro Horticulture Co, Seneca, Illinois, USA). Plants of each accession were thinned to three seedlings per pot, 1 wk after emergence. Pots were randomly assigned positions in a greenhouse. An automated drip irrigation system was used to deliver nutrient solution to each pot, three times a day. Sufficient solution was delivered to ensure soil saturation; excess solution was allowed to drain from the pots. The nutrient solution contained the following concentrations of mineral salts: 1.0 mM KNO3, 0.4 mM Ca(NO3)2, 0.1 mM MgSO4, 0.15 mM KH2PO4, 25 ?M CaCl2, 25 ?M H3BO3, 2 ?m MnSO4, 2 ?M ZnSO4, 0.5 ?M CuSO4, 0.5 ?M H2MoO4, 0.1 ?M NiSO4, and 1 ?M Fe(?)-N, N?-ethylenebis[2-(2-hydroxyphenyl)-glycine] (Sprint 138; Becker-Underwood, Inc., Ames, Iowa, USA).
More information on Grusak's sampling and Experimental Design(a .doc file).
A Excel files with the 2009 mineral data .
Pea enation mosaic virus(PEMV) results. Data taken 32-34 days after inoculated.
Lentil accessions were planted during the last week of May using a hand-pushed belt planter. One row plots about three meters long and 75 cm between rows were established. Pea cultivars Little Marvel and Sounder were included periodically (about every 100 lentil accessions) as susceptible checks, and Oregon Sugar Pod II, Oregon Pioneer, and Oregon Trail were included as resistant checks. Moisture was provided through sprinkler irrigation irrigation applied at weekly intervals at the beginning of the growing season. After flowering, one final irrigation was applied. We relied upon natural infection (via aphids) from the surrounding vegetation. Plots were rated during pod fill using a 1 - 5 scale where 1 = plants healthy (leaves green and fully expanded, normal leaf and stem shape, normal growth), 3 = plants with moderate infection (leaves may have chlorotic patches, leaves and stems with distorted growth, some stunting), and 5 = plants severely stunted, distorted and dead.
Lentil accessions were planted during the last week of May using a hand-pushed belt planter. One row plots about three meters long and 75 cm between rows were established. Pea cultivars Little Marvel and Sounder were included periodically (about every 100 lentil accessions) as susceptible checks, and Oregon Sugar Pod II, Oregon Pioneer, and Oregon Trail were included as resistant checks. Moisture was provided through sprinkler irrigation irrigation applied at weekly intervals at the beginning of the growing season. After flowering, one final irrigation was applied. We relied upon natural infection (via aphids) from the surrounding vegetation. Plots were rated during pod fill using a 1 - 5 scale where 1 = plants healthy (leaves green and fully expanded, normal leaf and stem shape, normal growth), 3 = plants with moderate infection (leaves may have chlorotic patches, leaves and stems with distorted growth, some stunting), and 5 = plants severely stunted, distorted and dead.
Lentil accessions were planted during the last week of May using a hand-pushed belt planter. One row plots about three meters long and 75 cm between rows were established. Pea cultivars Little Marvel and Sounder were included periodically (about every 100 lentil accessions) as susceptible checks, and Oregon Sugar Pod II, Oregon Pioneer, and Oregon Trail were included as resistant checks. Moisture was provided through sprinkler irrigation irrigation applied at weekly intervals at the beginning of the growing season. After flowering, one final irrigation was applied. We relied upon natural infection (via aphids) from the surrounding vegetation. Plots were rated during pod fill using a 1 - 5 scale where 1 = plants healthy (leaves green and fully expanded, normal leaf and stem shape, normal growth), 3 = plants with moderate infection (leaves may have chlorotic patches, leaves and stems with distorted growth, some stunting), and 5 = plants severely stunted, distorted and dead.
Click for an Excel spread sheet on the 2003 PEMV data (.xls).
Lentil accessions were planted during the last week of May using a hand-pushed belt planter. One row plots about three meters long and 75 cm between rows were established. Pea cultivars Little Marvel and Sounder were included periodically (about every 100 lentil accessions) as susceptible checks, and Oregon Sugar Pod II, Oregon Pioneer, and Oregon Trail were included as resistant checks. Moisture was provided through sprinkler irrigation irrigation applied at weekly intervals at the beginning of the growing season. After flowering, one final irrigation was applied. We relied upon natural infection (via aphids) from the surrounding vegetation. Plots were rated during pod fill using a 1 - 5 scale where 1 = plants healthy (leaves green and fully expanded, normal leaf and stem shape, normal growth), 3 = plants with moderate infection (leaves may have chlorotic patches, leaves and stems with distorted growth, some stunting), and 5 = plants severely stunted, distorted and dead.
Click for an Excel spread sheet on the 2004 PEMV data (.xls).
Lentil accessions were planted during the last week of May using a hand-pushed belt planter. One row plots about three meters long and 75 cm between rows were established. Pea cultivars Little Marvel and Sounder were included periodically (about every 100 lentil accessions) as susceptible checks, and Oregon Sugar Pod II, Oregon Pioneer, and Oregon Trail were included as resistant checks. Moisture was provided through sprinkler irrigation irrigation applied at weekly intervals at the beginning of the growing season. After flowering, one final irrigation was applied. We relied upon natural infection (via aphids) from the surrounding vegetation. Plots were rated during pod fill using a 1 - 5 scale where 1 = plants healthy (leaves green and fully expanded, normal leaf and stem shape, normal growth), 3 = plants with moderate infection (leaves may have chlorotic patches, leaves and stems with distorted growth, some stunting), and 5 = plants severely stunted, distorted and dead.
Click for an Excel spread sheet on the 2005 PEMV data (.xls).
Selected seeds of a single seed phenotype within each accession used previously in the mineral nutrient study of the Cicer collection (Grusak, 2005, GRIN) were used for this analysis. Protein concentration (N H 5.5) of the ground seed was determined using a Leco nitrogen analyzer (Leco Corp., St Joseph, MI, USA).
Pea seed-borne mosaic virus (PSbMV) is not considered a problem in Australian lentils as no seed transmission in commercial lentil seed lots has so far been reported. PSbMV lentil-seed transmissible strains were found in accessions from the Australian germplasm collection that were imported from the USDA germplasm collection and were previously reported to contain PSbMV.
100 seed weight at the Western Regional Plant Introduction Station, Pullman Washington, USA
The National Plant Germplasm System (NPGS) identifies there accssions with an accession prefix (ACP) and a accession number (ACNO) and a accessions suffix (ACS). When single plant accessions were developed for Lens a accession suffix was added of 'LSP' to the new accession.. The accession prefix and accession number are the same as the accession the single plant came from.
Singe plant derived accessions were developed for an association mapping population. They are currently unavailable.
Images taken at Whitlow farm at Pullman Washington
Images taken at Whitlow farm at Pullman Washington