Evaluation location: Texas, United States
Plants (Lentil ? Single Plant Core) were grown in 5-liter black plastic pots filled with a 2:1 (vol:vol) mixture of synthetic soil (Metro-Mix 360; Scotts-Sierra Horticultural Products Co., Marysville, Ohio, USA) and vermiculite (Strong-Lite Medium Vermiculite, Sun Gro Horticulture Co, Seneca, Illinois, USA). Plants of each accession were thinned to three seedlings per pot, 1 wk after emergence. Pots were randomly assigned positions in a greenhouse. An automated drip irrigation system was used to deliver nutrient solution to each pot, three times a day. Sufficient solution was delivered to ensure soil saturation; excess solution was allowed to drain from the pots. The nutrient solution contained the following concentrations of mineral salts: 1.0 mM KNO3, 0.4 mM Ca(NO3)2, 0.1 mM MgSO4, 0.15 mM KH2PO4, 25 ?M CaCl2, 25 ?M H3BO3, 2 ?m MnSO4, 2 ?M ZnSO4, 0.5 ?M CuSO4, 0.5 ?M H2MoO4, 0.1 ?M NiSO4, and 1 ?M Fe(?)-N, N?-ethylenebis[2-(2-hydroxyphenyl)-glycine] (Sprint 138; Becker-Underwood, Inc., Ames, Iowa, USA).
More information on Grusak's sampling and Experimental Design(a .doc file).
A Excel files with the 2009 mineral data .