| 0 | PI 701410 | 'LD19-APH01' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140483 | PI 701410 |
| 1 | PI 701411 | 'LD19-APH02' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140484 | PI 701411 |
| 2 | PI 701412 | 'LD19-APH03' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140485 | PI 701412 |
| 3 | PI 701413 | 'LD19-APH04' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140486 | PI 701413 |
| 4 | PI 701414 | 'LD19-APH05' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140487 | PI 701414 |
| 5 | PI 701415 | 'LD19-APH06' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140488 | PI 701415 |
| 6 | PI 701416 | 'LD19-APH07' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140489 | PI 701416 |
| 7 | PI 701417 | 'LD19-APH08' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140490 | PI 701417 |
| 8 | PI 701418 | 'LD19-APH09' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140491 | PI 701418 |
| 9 | PI 701419 | 'LD19-APH10' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140492 | PI 701419 |
| 10 | PI 701420 | 'LD19-APH11' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140493 | PI 701420 |
| 11 | PI 701421 | 'LD19-APH12' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140494 | PI 701421 |
| 12 | PI 701422 | 'LD19-APH13' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140495 | PI 701422 |
| 13 | PI 701423 | 'LD19-APH14' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140496 | PI 701423 |
| 14 | PI 701424 | 'LD19-APH15' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140497 | PI 701424 |
| 15 | PI 701425 | 'LD19-APH16' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140498 | PI 701425 |
| 16 | PI 701426 | 'LD19-APH17' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140499 | PI 701426 |
| 17 | PI 701427 | 'LD19-APH18' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140500 | PI 701427 |
| 18 | PI 701428 | 'LD19-APH19' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140501 | PI 701428 |
| 19 | PI 701429 | 'LD19-APH20' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140502 | PI 701429 |
| 20 | PI 701430 | 'LD19-APH21' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140503 | PI 701430 |
| 21 | PI 701431 | 'LD19-APH22' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140504 | PI 701431 |
| 22 | PI 701432 | 'LD19-APH23' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140505 | PI 701432 |
| 23 | PI 701433 | 'LD19-APH24' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140506 | PI 701433 |
| 24 | PI 701434 | 'LD19-APH25' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140507 | PI 701434 |
| 25 | PI 701435 | 'LD19-APH26' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140508 | PI 701435 |
| 26 | PI 701436 | 'LD19-APH27' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140509 | PI 701436 |
| 27 | PI 701437 | 'LD19-APH28' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140510 | PI 701437 |
| 28 | PI 701438 | 'LD19-APH29' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140511 | PI 701438 |
| 29 | PI 701439 | 'LD19-APH30' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140512 | PI 701439 |
| 30 | PI 701440 | 'LD19-APH31' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140513 | PI 701440 |
| 31 | PI 701441 | 'LD19-APH32' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar Titan. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, tawny pubescence, brown pods at maturity, and yellow seed with black hila. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140514 | PI 701441 |
| 32 | PI 701442 | 'LD19-APH33' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140550 | PI 701442 |
| 33 | PI 701443 | 'LD19-APH34' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140551 | PI 701443 |
| 34 | PI 701444 | 'LD19-APH35' | Glycine max (L.) Merr. | Illinois, United States | SOY | | | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140552 | PI 701444 |
| 35 | PI 701445 | 'LD19-APH36' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140553 | PI 701445 |
| 36 | PI 701446 | 'LD19-APH37' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140554 | PI 701446 |
| 37 | PI 701447 | 'LD19-APH38' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140555 | PI 701447 |
| 38 | PI 701448 | 'LD19-APH39' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140556 | PI 701448 |
| 39 | PI 701449 | 'LD19-APH40' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140557 | PI 701449 |
| 40 | PI 701450 | 'LD19-APH41' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140558 | PI 701450 |
| 41 | PI 701451 | 'LD19-APH42' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140559 | PI 701451 |
| 42 | PI 701452 | 'LD19-APH43' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140560 | PI 701452 |
| 43 | PI 701453 | 'LD19-APH44' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140561 | PI 701453 |
| 44 | PI 701454 | 'LD19-APH45' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140562 | PI 701454 |
| 45 | PI 701455 | 'LD19-APH46' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140563 | PI 701455 |
| 46 | PI 701456 | 'LD19-APH47' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140564 | PI 701456 |
| 47 | PI 701457 | 'LD19-APH48' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140565 | PI 701457 |
| 48 | PI 701458 | 'LD19-APH49' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140566 | PI 701458 |
| 49 | PI 701459 | 'LD19-APH50' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140567 | PI 701459 |
| 50 | PI 701460 | 'LD19-APH51' | Glycine max (L.) Merr. | Illinois, United States | SOY | | | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140568 | PI 701460 |
| 51 | PI 701461 | 'LD19-APH52' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140569 | PI 701461 |
| 52 | PI 701462 | 'LD19-APH53' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140570 | PI 701462 |
| 53 | PI 701463 | 'LD19-APH54' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140571 | PI 701463 |
| 54 | PI 701464 | 'LD19-APH55' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140572 | PI 701464 |
| 55 | PI 701465 | 'LD19-APH56' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140573 | PI 701465 |
| 56 | PI 701466 | 'LD19-APH57' | Glycine max (L.) Merr. | Illinois, United States | SOY | | | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140574 | PI 701466 |
| 57 | PI 701467 | 'LD19-APH58' | Glycine max (L.) Merr. | Illinois, United States | SOY | | | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140575 | PI 701467 |
| 58 | PI 701468 | 'LD19-APH59' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140576 | PI 701468 |
| 59 | PI 701469 | 'LD19-APH60' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140577 | PI 701469 |
| 60 | PI 701470 | 'LD19-APH61' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140578 | PI 701470 |
| 61 | PI 701471 | 'LD19-APH62' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140579 | PI 701471 |
| 62 | PI 701472 | 'LD19-APH63' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140580 | PI 701472 |
| 63 | PI 701473 | 'LD19-APH64' | Glycine max (L.) Merr. | Illinois, United States | SOY | | Not Available | 2022 | DEVELOPED | | | | | | | The soybean aphid, Aphis glycines (Matsumura), resistance genes Rag1, Rag2, Rag3, Rag4, and Rag6 were backcrossed into the background of the MG II soybean cultivar LD02-4485. Each gene was first backcrossed four times into this background and then stacked through crossing the backcross lines together. The sources of the resistance genes are ‘Dowling’ (PI 548663) for Rag1, ‘Sugao Zarai’ (PI 200538) for Rag2, PI 567543C for Rag3, PI 567541B for rag4, and wild soybean Glycine soja 85-32, for Rag6. From these crosses, an F1 heterozygous for all five resistance genes was developed and F3 plants homozygous for all 32 combinations of these resistance genes were identified. The backcross lines match the recurrent parent with purple flowers, grey pubescence, brown pods at maturity and yellow seed. Hila color varied with 13 lines having buff hila that matched the recurrent parent, 11 with imperfect black and eight with a mixture of buff and imperfect black. The recurrent parent and backcross lines all have indeterminate growth habit. The lines do not carry any transgenes. The backcross lines were evaluated for agronomic traits and were evaluated for resistance to four soybean aphid biotypes. There were significant (P<0.05) differences in the performance of the backcross lines for agronomic traits, however, the grain yield among most backcross lines did not significantly differ from the recurrent parent. There were significant differences among lines for the number of aphids on plants after inoculation with each aphid biotype. Those lines with the Rag6 resistance gene and many gene pyramids showed a high level of resistance to all four aphid biotypes. Because these backcross lines have the resistance genes in a mostly uniform background, they will be a useful source of germplasm for researchers studying soybean aphid isolates, the interaction between resistance genes and aphid isolates, and for breeders who are developing germplasm with resistance gene pyramids. | 2140581 | PI 701473 |