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ACCESSIONPLANT NAMETAXONOMYORIGINGENEBANKIMAGEAVAILABILITYRECEIVEDSOURCE TYPESOURCE DATECOLLECTION SITECOORDINATESELEVATIONHABITATIMPROVEMENT LEVELNARRATIVE
0PI 698814Kronos-ful2-nullTriticum turgidum L. subsp. durum (Desf.) van Slageren California, United StatesNSGCKERNELS2021DEVELOPEDGenetic materialWild type Kronos (PI 576168) is a high yielding durum wheat line with a spring growth habit and excellent pasta quality. A derived line carrying loss-of-function mutations in both VRN2 homeologs was described before (Distelfeld et al. 2009), and was used as recurrent parent. Both Kronos and Krons-vrn2-null have functional copies of the FUL-A2 and FUL-B2 genes. The ful2-null mutant combines mutations ful-A2 (Kronos T4-837) and ful-B2 (Kronos T4-2911). The ful-A2 mutant has a mutation in the splice donor site of the fifth intron. RT-PCR and sequencing analysis of the ful-A2 transcripts revealed two incorrect splicing forms one with a deletion of 14 amino acids in the middle of the K-box (from position 144 to 157) and the other with a premature stop codon. The ful-B2 mutant (Kronos T4-2911) carries a premature stop codon at position 162 (Q162*) that removed the last 13 amino acids of the K-box and the entire Cterminus. The ful2-null mutant shows a significant increase in the number of spikelets per spike and heads at the same time as Kronos-vrn2-null. This mutant and its combinations with vrn1-null and ful3-null are described in more detail in Li et al. 2019.2116454PI 698814
1PI 698815Kronos-ful3-nullTriticum turgidum L. subsp. durum (Desf.) van Slageren California, United StatesNSGCKERNELS2021DEVELOPEDGenetic materialWild type Kronos (PI 576168) is a high yielding durum wheat line with a spring growth habit and excellent pasta quality. A derived line carrying loss-of-function mutations in both VRN2 homeologs was described before (Distelfeld et al. 2009), and was used as recurrent parent. Both Kronos and Krons-vrn2-null have functional copies of the FUL-A3 and FUL-B3 genes. The ful3-null mutant combines mutations FUL-A3 (Kronos T4-2375) and FUL-B3 (Kronos T4-2139). The FUL-A3 mutant carries a mutation in the splice acceptor site of the third intron, which generates a new splice acceptor site, a shift in the reading frame and a premature stop codon. The FUL-B3 mutant carries a premature stop codon at amino acid position 132 (Q132*) that removes half of the K-box and the complete C-terminus. The ful3-null mutant did not show any evident phenotype when present alone. When ful3-null was combined with vrn1-null and ful2-null, the triple mutant showed reduced stem length and increases vegetative characteristics of the tiller-like lateral organs in the spike (Li et al. 2019).2116455PI 698815