Methods
In 2013, Jeffrey D. Carstens (North Central Regional Plant Introduction Station) and Andrew P. Schmitz completed an expedition throughout Kansas, Missouri, Illinois, Indiana, and Kentucky to document the natural occurrence of Gymnocladus dioicus along with notes concerning its habitat and associated species, to make representative seed collections, and ultimately to make germplasm available for research.
Germinations are performed at 20C/30C alternating temperatures with water as the medium. Fifty seeds are put on top of blotters for each of four replications. For Linum species other than usitatissimum, the number of germinated, abnormal, and dormant seeds are counted at 7, 10, and 14 days after placing on blotters. Linum usitatissimum accessions are counted at 3 and 7 days after placing on blotters. >6000 seed pull 200 seed (4x50). <6000 >2100 seed pull 100 seed (4x25). <2100 seed pull no seed for germiation test.
Fifty/twenty five seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Germinations are performed in a high humidity germinator set at 12 hours 20 degrees centigrade in the dark alternating with 12 hours 30 degrees centigrade in the light. Towels are kept moist with distilled water during the germination period. The number of germinated, abnormal and hard seed are counted at 5 and 14 days after the germinations are established. >1500 seed pull 200 seed. >1200 seed < 1500 seed pull 100 seed. <1200 seed pull no seed.
Comment: The data in this study were recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Laura Marek (515) 294-3519.
Year tested: 1950 Comment: North Central Regional PI Station in Ames, Iowa staff recorded data. For additional information, contact Laura Fredrick Marek (515-294-3519).
Year tested: 1960 Comment: North Central Regional PI Station in Ames, Iowa staff recorded data. For additional information, contact Laura Fredrick Marek (515) 294-3519.
Year tested: //1961 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1962 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1963 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1964 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1965 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1966 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1967 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1968 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1969 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1970 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1971 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1972 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1973 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1974 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1975 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1976 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1977 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1978 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1979 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Year tested: //1980 Comment: The data in this study was recorded by the staff of the North Central Regional PI Station in Ames, Iowa. For additional information, contact Mary Brothers at (515) 294-6507.
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 minutes W Latitude).
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 minutes W Latitude).
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 minutes W Latitude).
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Cult plants grown to maturity in 5 gal pots. Bonzi soil drench at V4 stage.
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, PI farm
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance in Farm GH-1, NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
sunflower descriptor data recorded during collection maintenance at NCRPIS, Ames, Ia
sunflower descriptor data recorded during collection maintenance at NCRPIS, Ames, Ia
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS.
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Sunflower descriptor data recorded during collection maintenance at the NCRPIS, Ames, IA
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1960
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1961
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1961
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1962
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1962
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1963
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1963
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1964
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1964
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1965
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1965
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1966
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1966
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1967
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1967
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1968
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1968
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1969
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1969
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1970
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1970
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1971
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1971
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1972
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1972
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1973
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1973
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1974
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1974
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1975
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1975
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1976
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1976
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1977
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1977
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1978
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1978
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1979
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1979
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1980
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1980
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1981
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1981
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1983
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1984
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1985
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1986
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1987
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1988
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1988
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1989
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1989
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1990
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1990
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1991
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field FIELD Exp. Location: PI FARM Latitude: 42 Degrees 0 Minutes N Longitude: 93 Degrees 30 Minutes W Year planted: //1991
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance in Farm GH-3, PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 minutes W Latitude).
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Sunflower descriptor data recorded during collection maintenance at the PI farm (42 degrees 0 minutes N Longitude, 93 degrees 30 Minutes W Latitude.)
Study Name: Sunflower Descriptors Obtained During Collection Maintenance Experiment Type: Field Laboratory Exp. Location: PI Farm Comment: Evaluation data on original seed samples.
Sunflower 100 seed weight data obtained during seed storage
Cultivated sunflower core of 112 sunflower accessions. SAS's FASTCLUS procedure was used to perform a disjoint cluster analysis based on Euclidean distances calculated from 20 sunflower descriptors. See Brothers and Miller .
Active accessions (11-20-06) in the NC7-sun.cults maintenance group to allow a descriptor (IMPROVSTAT) to differentiate between cultivated and wild H. annuus on public GRIN.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB GH Exp. Design: RB Comment: Evaluation for resistance to Downey Mildew Race 4. per accession.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB GH Exp. Design: RB Comment: Evaluation for resistance to Downey Mildew Race 2. Percentages were based on a maximum of 60 plants per accession.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB GH Exp. Design: RB Comment: Evaluation for resistance to Downey Mildew Race 3. Percentages were based on a maximum of 60 plants per accession.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB GH Exp. Design: RB Comment: Evaluation for resistance to Downey Mildew Race 4. Percentages were based on a maximum of 60 plants per accession.
Entries were planted in single row plots, 22' long, on 30" centers, with three replications at St. Hilaire, MN. No artificial inoculations were made but the field was in an area known to have severe Phomopsis the previous year. Counts of diseased plants were made on Sept. 11 and Sept. 24, or approximately 4 weeks post bloom and at physiological maturity of most entries. The second disease rating (Sept. 24) was recorded for plots which had < 20% disease at the first rating. The simple, total percentage of Phomopsis infected plants includes plants with any Phomopsis symptoms. Disease data on late-maturing entries, however, should be viewed with caution as there was not sufficient growing season for adequate disease development on these accessions.
Entries were planted in single row plots, 22' long, on 30" centers, with three replications at Thief River Falls, MN. No artificial inoculations were made but the field was in an area known to have severe Phomopsis the previous year. Counts of diseased plants were made on Sept. 17 and Oct. 2, or approximately 4 weeks post bloom and at physiological maturity of most entries. The second disease rating (Oct. 2) was recorded for plots which had < 20% disease at the first rating. The simple, total percentage of Phomopsis infected plants includes plants with any Phomopsis symptoms. Disease data on late-maturing entries, however, should be viewed with caution as there was not sufficient growing season for adequate disease development on these accessions.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB GH Exp. Design: RB Exp. Location: Fargo
Accessions were tested under greenhouse conditions by inoculating three-week old seedlings. Individual plants were inoculated with rust races 1-4 using the Q-tip method. Plants were evaluated using the classical rust severity scale of `0-4'. Pustules classified as 0, 1 or 2 were categorized as resistant and type 3 and 4 pustules were lumped into the susceptible category.
Fifteen plants (three replicates of five plants) were inoculated with race 3 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant. For accessions classified as resistant 15 plants (in some cases more than 15) were retested. The total number of plants tested for the accessions with a high percentage of resistant plants is between 30 and 40 plants.
Fifteen plants (three replicates of five plants) were inoculated with race 3 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant.
Fifteen plants (three replicates of five plants) were inoculated with race 3 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant. For accessions classified as resistant 15 plants (in some cases more than 15) were retested. The total number of plants tested for the accessions with a high percentage of resistant plants is between 30 and 40 plants.
Fifteen plants (three replicates of five plants) were inoculated with race 4 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant. For accessions classified as resistant 15 plants (in some cases more than 15) were retested. The total number of plants tested for the accessions with a high percentage of resistant plants is between 30 and 40 plants.
Fifteen plants (three replicates of five plants) were inoculated with race 4 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant.
Fifteen plants (three replicates of five plants) were inoculated with race 4 rust spores mixed with Soltrol 160 mineral oil. Plants were evaluated on the basis on percent leaf area covered with pustules and grouped into the following categories: 0, 0.1, 0.2, 1.5, 1.0, 2.0, and 5.0% leaf area covered. Plants with 0.5% or less of the foliage covered by rust were classified as resistant. For accessions classified as resistant 15 plants (in some cases more than 15) were retested. The total number of plants tested for the accessions with a high percentage of resistant plants is between 30 and 40 plants.
Data presented are the mean results (percent resistant plants showing no symptom of wilting and/or a basal stem rot lesion) from a two year (2008, 2009), three location (Crookston, MN, 2008, 2009; Davenport, ND, 2008; Grandin, ND, 2009) field trial for 259 PIs. The trials were conducted using sets-within-replications experimental design with two replications in each location (13 sets in each replication). Commercial hybrids `Croplan 305?, resistant, and `Cargill 270?, susceptible, were used as checks in all sets. Seeds were planted in 6 m long single-row plots with 75 cm row spacing, yielding approximately 25 plants per row. S. sclerotiorum inoculum consisting of mycelium on millet was applied in each trial when the plants were approximately V-6 growth stage, or no more than 45 cm tall (5 to 6 weeks after planting). 2008 susceptible check mean across sets and locations: 44.00; 2009 susceptible check mean across sets and locations: 78.24. 2008 resistant check: mean across sets and locations 75.22; 2009 resistant check mean across sets and locations: 79.65.
1986 Sclerotinia evaluation data. Natural infection of Sclerotinia stalk and head rot, in Moorhead, MN nursery. Three replicates of date. Numbers are % infection with hybrid 894 and RHA 801 as checks. Original values of controls: HYB 894 -SCLERMSROT 19%, SCLERHDROT 25%; RHA 801 - SCLERMSROT 2.4%, SCLERHDROT 13%.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB FIELD Exp. Design: RCB Comment: Randomized complete block design with 4 replications. Original value of hybrid 894 (control) = 82% resistant.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB FIELD Exp. Design: RBC Comment: Randomized complete block design with 4 replications. Original value of hybrid 894 (control) = 81% resistant.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB FIELD Exp. Design: RCB Comment: Randomized complete block design with 4 replications. Original value of hybrid 894 (control) = 51% resistant.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB FIELD Exp. Design: RCB Comment: Randomized complete block design with 4 replications. Original value of hybrid 894 (control) = 61% resistant.
Study Name: Sunflower Disease Evaluation Experiment Type: RCB FIELD Exp. Design: RCB Comment: Randomized complete block design with 4 replications. Original value of hybrid 894 (control) = 84% resistant.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field Year started: //1986 Year seeded: //1986 Year tested: //1986 Comment: Usually 2 plants were sampled per plot with up to 4 replications. No control values were submitted with this data.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Exp. Location: Prosper, ND Year started: //1987 Year seeded: //1987 Year tested: //1987 Comment: Usually two plants were sampled per plot with up to 4 replications. The number of reps were low in some cases due to hail damage. Hybrid '894' had a raw Banded SF Moth damage score = 19.3% (average of 8 plots).
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Comment: Usually two plants were sampled per plot with up to 4 replications. Hybrid '894' (control) had a raw Banded SF Moth score = 1.4% (average over 8 plots). For wild accessions evaluautions are made on the number of larvae per plant relative to the test average.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Comment: Usually 2 plants were sampled per plot with up to 4 replicat replications. Hybrid '894' (control) had a raw Banded SF Moth percentage = 2.1% (average over 7 plots). For wild accessions evaluations are made on the number of larvae per plant relative to the test average.
Testing is conducted using a randomized block design with up to four replications. Banded Sunflower Moth data for cultivated accessions are in terms of number of larvae per plant (orgvalue) and the number of larvae per plant relative to that on the check (Hybrid 894, Ames-7576). Data for wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Banded Sunflower Moth data for cultivated accessions are in terms of number of larvae per plant (orgvalue) and the number of larvae per plant relative to that on the check (Hybrid 894, Ames-7576). Data for wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Banded Sunflower Moth data for cultivated accessions are in terms of number of larvae per plant (orgvalue) and the number of larvae per plant relative to that on the check (Hybrid 894, Ames-7576). Data for wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Banded Sunflower Moth data for cultivated accessions are in terms of number of larvae per plant (orgvalue) and the number of larvae per plant relative to that on the check (Hybrid 894, Ames-7576). Data for wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Banded Sunflower Moth data for cultivated accessions are in terms of number of larvae per plant (orgvalue) and the number of larvae per plant relative to that on the check (Hybrid 894, Ames-7576). Data for wild accessions are given relative to the test average rather than relative to 894.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Year started: //1986 Year seeded: //1986 Year tested: //1986 Comment: Usually 2 plants were sampled per plot with up to 4 replications. No control values were submitted with data.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field Year started: //1987 Year seeded: //1987 Year tested: //1987 Comment: Usually two plants were sampled per plot with up to 4 replications. Hybrid '894' (control) had a raw Red SF Weevil emergence score = 43.4 (average over 10 plots).
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Comment: Usually two plants were sampled per plot with up to 4 replications. Hybrid '894' had a Red SF Weevil emergence rating = 59.7% (average of 7 plots). Wild accession evaluations are made on the percentage of seed damaged by the RSSW relative to the test average. Wild sunflower are harvested prior to physiological maturity to ensure collection of seed.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB Comment: Usually two plants were sampled per plot with up to 4 replications. Hybrid '894' had a Red SF Weevil emergence rating = 30.6% (average of 7 plots). Wild accession evaluations are made on the percentage of seed damaged by the RSSW relative to the test average. Wild sunflower are harvested prior to physiological maturity to ensure collection of seed.
Testing is conducted using a randomized block design with up to four replications. Red Sunflower Seed Weevil data for cultivated accessions are the percentage of seeds per plant damaged by the RSSW (orgvalue) and the number damaged relative to that on the check (Hybrid 894). Data for the wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Red Sunflower Seed Weevil data for cultivated accessions are the percentage of seeds per plant damaged by the RSSW (orgvalue) and the number damaged relative to that on the check (Hybrid 894). Data for the wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Red Sunflower Seed Weevil data for cultivated accessions are the percentage of seeds per plant damaged by the RSSW (orgvalue) and the number damaged relative to that on the check (Hybrid 894). Data for the wild accessions are given relative to the test average rather than relative to 894.
Testing is conducted using a randomized block design with up to four replications. Red Sunflower Seed Weevil data for cultivated accessions are the percentage of seeds per plant damaged by the RSSW (orgvalue) and the number damaged relative to that on the check (Hybrid 894). Data for the wild accessions are given relative to the test average rather than relative to 894. A small set of accessions in test 9413 were found to have two morphological types. One type was consistently resistant to the rssw and the other type was susceptible. The accessions have been tested numerous times and have been consistently resistant, if the proper type was evaluated. The morphological type is indicated in the comment field of the observation records. The following accessions have information for two morphological types: PI 170411, PI 170417, PI 170424, PI 175730, PI 253417, and PI 480471.
Study Name: Sunflower Resistance to North Dakota Insects Experiment Type: Field FIELD Exp. Design: RB
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.erminated and field increase in Parlier. Sandy plots with irrigation, caging, pollination by bees. Heads processed in Parlier; seed re-cleaned in Ames.
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.erminated and field increase in Parlier. Sandy plots with irrigation, caging, pollination by bees. Heads processed in Parlier; seed re-cleaned in Ames.
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.erminated and field increase in Parlier. Sandy plots with irrigation, caging, pollination by bees. Heads processed in Parlier; seed re-cleaned in Ames.
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.erminated and field increase in Parlier. Sandy plots with irrigation, caging, pollination by bees. Heads processed in Parlier; seed re-cleaned in Ames.
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.erminated and field increase in Parlier. Sandy plots with irrigation, caging, pollination by bees. Heads processed in Parlier; seed re-cleaned in Ames.
Germinated in Ames. Transplanted to jiffy pots, shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads shipped to and seed processed in Ames.
Seedlings germinated in Ames. Transplanted to jiffy pots. Shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads with some stalk harvested, processed in Ames.
Seedlings germinated in Ames. Transplanted to jiffy pots. Greenhouse inspected by IDALS not to have invasive insects. Shipped to Parlier. Field increase in sandy plots with irrigation, caging, pollination by bees. Heads with some stalk harvested, processed in Ames.
Seedlings germinated in Ames and transplanted to containers with soiless mix. Greenhouse inspected by IDALS to be free from Japanese Beetles; Story County is quarantineed by CA for Japanese beetles. Shipped to NALGRU, Parlier, CA. Field increase in silty plots with irrigation, caging, pollination by added bees. Heads with some stalk harvested, shipped to and processed in Ames.
Seedlings germinated in Ames and transplanted to containers with soiless mix. Greenhouse inspected by IDALS to be free from Japanese Beetles; Story County is quarantineed by CA for Japanese beetles. Shipped to NALGRU, Parlier, CA. Field increase in silty plots with irrigation, caging, pollination by added bees. Heads with some stalk harvested, shipped to and processed in Ames.
Digital photo, capitulum in field, NCRPIS
Digital photo, leaf in field, NCRPIS
Digital photo, full plant in field, NCRPIS
Digital photo, full plant in field, NCRPIS
Three replications of each accession were planted January 15, 1997 at Potchefstroom, South Africa. The natural infection of Albugo tragopogonis was rated 1-9 for leaves (WHITERUST) and stems (ALBSTMSPOT) on a per row basis. A 1 and 3 indicated resistance, and 5, 7, and 9, susceptibility. Reported values are the means for the three replications.
Three replications of each accession were planted January 15, 1997 at Potchefstroom, South Africa. The natural infection of Albugo tragopogonis was rated 1-9 for leaves (WHITERUST) on a per row basis. A 1 and 3 indicated resistance, and 5, 7, and 9, susceptibility. Reported values are the means for the three replications.
Sample, bulk harvested/collected achenes, 200 dpi scan (3200 x 2400 pixels), NCRPIS.
Sample, bulk harvested/collected achenes, 400 dpi scan (3200 x 2400 pixels), NCRPIS.
Total, bulk harvested/collected achenes, 200 dpi scan (3200 x 2400 pixels), NCRPIS.
Total, bulk harvested/collected achenes, 400 dpi scan (3200 x 2400 pixels), NCRPIS.
Accessions were first screened using a refractometer. Accessions which had low refractive index values (indicative of high oleic acid content) were then screened using G.C. analysis to determine fatty acid content. Oleic and linoleic acid contents were estimated for all accessions using the following formulas: Linoleic Acid % = 9601.0 (RI) + 3.213 (T) - 14159.5 Oleic Acid % = -9474.0 (RI) - 3.170 (T) + 14059.9 Where: RI = refractive index value and T = 21 degrees Centigrade. From Goss, D.W. 1978. Estimating the linoleic and oleic acid contents of sunflower oil by refractive index. Proceedings of the eigth international sunflower conference: 564-569.
Fatty acid concentrations of original seed from 2002 exploration. Oil was solvent extracted from10-20 seeds using organic base-catalyzed transesterification of the triacylglycerols and gas chromatography with a capillary column, SP-2330, with flame ion detection (FID) and an electronic integrator
Fatty acid concentrations of accessions from 2005 explorations. Analyses were performed in 2006 using original seeds. Solvent extracted oil from 10 seeds using organic base-catalyzed transesterification of the triacylglycerols and gas chromatography with a capillary column, SP-2330, with flame ion detection (FID) and an electronic integrator.
Fatty acid concentrations of wild accessions from the Bushland Collection 1976-1980. All analyses performed in 1982 on seeds from original population. Solvent extracted oil from 10-20 seeds using organic base-catalyzed transesterification of the triacylglycerols and gas chromatography with a capillary column, SP-2330, with flame ion detection (FID) and an electronic integrator.
Fatty acid concentrations of accessions from 1989 exploration. Analyses were performed in 1989 on seeds from the original population. Solvent extracted oil from 10-20 seeds using organic base-catalyzed transesterification of the triacylglycerols and gas chromatography with a capillary column, SP-2330, with flame ion detection (FID) and an electronic integrator.
Fatty acid concentrations of accessions from 1991 exploration. Analyses were performed in 1991 on seeds from the original population. Solvent extracted oil from 10-20 seeds using organic base-catalyzed transesterification of the triacylglycerols and gas chromatography with a capillary column, SP-2330, with flame ion detection (FID) and an electronic integrator.
Materials and methods followed that of Cronn et al, TAG(1997) 95:532-545. Starch gel electrophoresis was performed on crude protein extracts of cotyledon tissue. Approximately 20 plants were analyzed per accession (number of plants is recorded in sample size). Tissue was homoginized in a 1.5 ml centrifuge tube with a power-driven acetal pestle (on ice) in solubilization buffer [75 mM sodium phosphate (pH 7.5), 100 mM sodium ascorbate, 10 mM sodium diethyldithiocarbamate, 10 mM dithioerythritol, 10% (w/v) PVP-40, 5% sucrose, 0.5% bovine serum albumin and 10 mM B-mercaptoethanol]. Extracts were frozen at -70 C until use. Enzymes were separated on 12% starch gels and were visualized. Refer to Cronn et al. for allele and locus designation.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
Study Name: Sunflower Oil % Analysis Experiment Type: Laboratory Laboratory Exp. Design: Single Rep Exp. Location: Fargo, ND Comment: Non-destructive NMR evaluation of distribution lots for oil percentage.
Study Name: Sunflower Oil % Analysis Experiment Type: Laboratory Laboratory Exp. Design: Single Rep Exp. Location: Fargo, N Dak Comment: Non-destructive NMR evaluation of distribution lots for oil percentage.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 30 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
Oil concentration of accessions from 2002 exploration. Analyses were performed in 2002 on seeds from the original population. Nuclear Magnetic Resonance (NMR) using 2 ml of dried seeds from wild Helianthus species (cultivated H. annuus uses 40 ml). This methodology is an acceptable technique for analysis. Oil concentration is expressed on a percent of dry weight basis.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
Oil concentration for wild accessions from the Bushland Collection 1976-1980. All analyses performed in 1982 on seeds from original population. Nuclear Magnetic Resonance (NMR) using 2 ml of dried seeds from wild Helianthus species (cultivated H. annuus uses 40 ml). This methodology is an acceptable technique for analysis. Oil concentration is expressed on a percent of dry weight basis.
Oil concentration of accessions from 1989 exploration. Analyses were performed in 1989 on seeds from the original population. Nuclear Magnetic Resonance (NMR) using 2 ml of dried seeds from wild Helianthus species (cultivated H. annuus uses 40 ml). This methodology is an acceptable technique for analysis. Oil concentration is expressed on a percent of dry weight basis.
Oil concentration of accessions from 1991 exploration. Analyses were performed in 1991 on seeds from the original population. Nuclear Magnetic Resonance (NMR) using 2 ml of dried seeds from wild Helianthus species (cultivated H. annuus uses 40 ml). This methodology is an acceptable technique for analysis. Oil concentration is expressed on a percent of dry weight basis.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
A 6 ml sample was analyzed using an Oxford 4000 Nuclear Magnetic Resonance Analyzer. Calibration time was 20 seconds, repeated twice.
903 Helianthus accessions were screened for resistance/tolerance to Orobanche at the Thrace Agricultural Research Institute (TARI), Edirne, Turkey. Two replications of single-row plots (5.10 cm in length) were planted April 11, 1993. Rows were spaced 1 meter apart and hills were made within each row at a 30 cm spacing. Although the field was naturally infested, Orobanche inoculum was also applied. The inoculum was prepared with Orobanche seeds collected from sunflower fields in different parts of the Thrace region. The Orobanche seeds were mixed with fine sand in a 1:15 ratio. Approximately 500 mg of inoculum was applied to each hill along with 3 sunflower seeds and the hill was irrigated. After emergence, plants were thinned to one sunflower plant per hill. Just prior to sunflower flowering, Orobanche shoots were beginning to emerge. Orobanche counts were taken prior to sunflower physiological maturity when the maximum number of Orobanche shoots was reached. Data were recorded for stand, number of sunflower plants parasitized by Orobanche, and total number of Orobanche shoots per plot. Frequency (% sunflower plants parasitized) and attacking rate (average number of Orobanche per parasitized sunflower) were calculated. Under Turkish conditions, sunflower varieties with a frequency < 10% and an attack rate < 1 are considered resistant or highly tolerant.
Study Name: Sunflower Phomopsis and Phoma Resistance Evaluation Experiment Type: Field FIELD Comment: Phomopsis and Phoma resistance scores taken on the same plots.
Aberdeen, Idaho, 1994. Planted June 8-10, 1994. Growth Habit readings taken in early August. Orders 7505, 7932, 8107.