HAZELNUT

DNA extracted from actively growing leaves. Individuals were amplified with PCR. PCR products were separated on Beckman CEQ. Markers were screened for genetic diversity.

Curator revision of core subset based on taxonomy, geographic origin, and unique traits.

Study Name: Corylus Field Evaluation Data 1989-1990 Experiment Type: Field FIELD Study Year: 1989 Year started: 09/01/1989 Year ended: 03/01/1990 Experiment length: 7 months

Study Name: Corylus observation data Experiment Type: Field FIELD Study Year: 1991

Study Name: Standard Filbert descriptors Experiment Type: Field FIELD Study Year: 1990

Study Name: Filbert descriptors 1990 Experiment Type: Field FIELD Study Year: 1990 Year started: 08/01/1990 Year ended: 03/01/1991 Experiment length: 1 year

Comment: These data are from several studies, 1988-1990

A 14 SSR multiplex fingerprinting set for Hazelnut identification was used in PCR reactions. All forward primers were labeled with the fluorescent dyes 6-FAM, NED and VIC. All reverse primers were PIG-tailed to have the consensus GTTT region attached to the 5 prime end of each reverse primer. TheType-it Microsatellite PCR Kit was used for amplification and PCR products were seperated on an Applied Biosystems (ABI) 3500 Genetic Analyzer

CEQ platform: Applied Biosystems (ABI) 3500 Genetic Analyzer