Evaluation location: Ontario, Canada
A 14 SSR multiplex fingerprinting set for Hazelnut identification was used in PCR reactions. All forward primers were labeled with the fluorescent dyes 6-FAM, NED and VIC. All reverse primers were PIG-tailed to have the consensus GTTT region attached to the 5 prime end of each reverse primer. TheType-it Microsatellite PCR Kit was used for amplification and PCR products were seperated on an Applied Biosystems (ABI) 3500 Genetic Analyzer
CEQ platform: Applied Biosystems (ABI) 3500 Genetic Analyzer