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ACCESSIONPLANT NAMETAXONOMYORIGINGENEBANKIMAGEAVAILABILITYRECEIVEDSOURCE TYPESOURCE DATECOLLECTION SITECOORDINATESELEVATIONHABITATIMPROVEMENT LEVELNARRATIVE
0PI 690471RMBHMTUP-C4Gossypium hirsutum L. Mississippi, United StatesCOTNot Available2019DEVELOPEDBreeding materialThe genetic base that is used for breeding upland cotton (Gossypium hirsutum L.) needs more allelic diversity. Use of wild species of Gossypium is one approach that is of interest to breeders, but interspecific crosses between G. hirsutum and wild tetraploid species or the cultivated tetraploid species G. barbadense L. have not been widely used in cultivar breeding due to the extreme difficulty of extracting elite lines in later generations. Following a non-conventional breeding approach, we used 32 chromosome substitution lines to capture allelic diversity from three donor tetraploid Gossypium species and combined that with the intra-specific allelic diversity among 5 Upland cultivars. By random-mating the 32 F1 hybrids and subsequent generations for 5 cycles, the intra- and inter-specific diversity from four Gossypium AD-genome species was combined in a new random-mated population dubbed "Random Mated Barbadense, Hirsutum, Mustelinum, Tomentosum, Upland Population" (RMBHMTUP-C4). This population is very diverse and should facilitate efforts to broaden the genetic base for cotton breeding. In spite of its allelic diversity, it has characteristics that make it very much like an upland cultivar population in phenotype and fiber properties. During the development of this population, we did not observe any the sterility or other breeding problems that are normally observed in conventional interspecific crosses. We harvested about 2,209 bolls after each of the five cycles of random mating (11,045 bolls). Assuming 25 seed per boll, this equates to 276,125 hand-crossed seed, each from union of two genetically unique pollen and egg gametes. Each of the corresponding 552,250 meioses that gave rise to these gametes would have included multiple opportunities for recombination of alleles. Thus, we have developed a unique random-mated population among four tetraploid Gossypium species.2091858PI 690471