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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 664300 | B2 | Nicotiana tabacum L. | Connecticut, United States | NLGRP | | Not Available | 2011 | DEVELOPED | | | | | | Cultivar | B2 is a male-sterile hybrid with resistance to four of the most common and destructive pathogens of tobacco in Connecticut, including Fusarium wilt, Tobacco mosaic virus (TMV), the tobacco cyst nematode(TCN) and blue mold. B2 was released by The Connecticut Agricultural Experiment Station in 2011. Initial crosses for cyst nematode resistance were made in 1987 between two flue-cured tobaccos, VA-81 and cultivar PD4 and three broadleaf inbreds resistant to Fusarium wilt and TMV. Selected F2 plants were backcrossed twice to CT broadleaf, and then inbred and selected over 12 generations using a bulk system of modified single seed descent in which only the top 2% of plants (20 plants of 1,000 grown) were selected for the next generation. Selection for TMV and TCN resistance was conducted in greenhouse screens. Lines were field selected for reduced sensitivity to weather fleck caused by ozone. An F12 selection was backcrossed again to the broadleaf cultivar `Scantic? inbred using pedigree selection over 10 generations with field and greenhouse selection. Progeny testing was performed to select F8 plants homozygous for G. t. tabacum resistance and the dominant hypersensitive gene for resistance to tobacco mosaic virus derived from Nicotiana glutinosa. A superior male-fertile inbred (MF-2) resulting from 27 generations of selection for broadleaf agronomic characteristics with 8 cycles of selection each for Fusarium wilt, TMV and TCN resistance was identified and crossed with a male-sterile CT broadleaf line with homozygous resistance to TMV and resistant to Fusarium wilt (MS-1) to produce the F1 male-sterile hybrid B2. Yield and quality characteristics of B2 were determined to be equal or superior to the current standard `C9?. | 1896752 | PI 664300 |