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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 639191 | WS4U | Panicum virgatum L. | Wisconsin, United States | S9 | | | 2005 | DEVELOPED | 07/18/2002 | | | | | Breeding material | Switchgrass seedlings from 135 germplasm sources were used, and 20 seedlings of each germplasm source were transplanted to three space planted nurseries. The germplasm sources consisted of 21 cultivars or experimental populations and 114 plant introductions from USDA-NPGS-GRIN. All but 13 of the plant introductions were collected from prairie remnants in eastern South Dakota. Plants were visually evaluated for vigor, lodging resistance, large stem size, and rust reaction. Two hundred fifty plants were selected from the three spaced-plant nurseries. One leaf per plant was analyzed for ploidy level determination using flow cytometry. Based on nuclear DNA content, plants were classified as tetraploid (n = 152) or octaploid (n = 98). Expected DNA content was 3.0 pg/nucleus for tetraploids (2n=4x=36), 4.5 pg/nucleus for hexaploids (2n=6x=54), and 6.0 pg/nucleus for octaploids (2n=8x=72). The use of DNA content for ploidy classification is less precise than chromosome counts and these classifications should not be taken as a warranty that all plants within each population have the designated euploid chromosome number, and the population may contain aneuploids and/or hexaploids. The plants classified as tetraploid were the parents of WS4U. | 1675937 | PI 639191 |
| 1 | PI 639192 | WS8U | Panicum virgatum L. | Wisconsin, United States | S9 | | | 2005 | DEVELOPED | 07/18/2002 | | | | | Breeding material | Switchgrass seedlings from 135 germplasm sources were used, and 20 seedlings of each germplasm source were transplanted to three space planted nurseries. The germplasm sources consisted of 21 cultivars or experimental populations and 114 plant introductions from USDA-NPGS-GRIN. All but 13 of the plant introductions were collected from prairie remnants in eastern South Dakota. Plants were visually evaluated for vigor, lodging resistance, large stem size, and rust reaction. Two hundred fifty plants were selected from the three spaced-plant nurseries. One leaf per plant was analyzed for ploidy level determination using flow cytometry. Based on nuclear DNA content, plants were classified as tetraploid (n = 152) or octaploid (n = 98). Expected DNA content was 3.0 pg/nucleus for tetraploids (2n=4x=36), 4.5 pg/nucleus for hexaploids (2n=6x=54), and 6.0 pg/nucleus for octaploids (2n=8x=72). The use of DNA content for ploidy classification is less precise than chromosome counts and these classifications should not be taken as a warranty that all plants within each population have the designated euploid chromosome number, and the population may contain aneuploids and/or hexaploids. The plants classified at octaploid were the parents of WS8U. | 1675938 | PI 639192 |