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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
|---|
| 0 | PI 610664 | UMN 3176 | Medicago sativa L. subsp. sativa | Minnesota, United States | W6 | | | 1999 | DEVELOPED | PRE 06/08/1999 | | | | | Breeding material | Ineffectively nodulated regenerating alfalfa germplasm developed to permit introduction of new traits into alfalfa by Agrobacterium-mediated transformation and regeneration of transgenic plants in tissue culture in a genetic background that is incapable of utilizing nitrogen (N) from the atmosphere through symbiotic N2-fixation. Ineffective Agate and Ineffective Saranac plants were crossed onto 68 clones of a regenerating genotype Regen-SY to produce the SYN1. All SYN1 progeny of both crosses were effectively nodulated. Approx. 210 SYN1 plants were randomly intercrossed to produce SYN2 seed. The SYN2 progeny segregated 2970 nodulated to 280 (8.6%) ineffectively nodulated plants. Approx. 59% (56/95) of the ineffective plants regenerated from leaf tissue. Plants which regenerated and were ineffectively nodulated were intercrossed to produce SYN3 seed (UMN 3176) for distribution. The SYN3 population had 98% ineffectively nodulated and 55% regenerating plants. | 1580239 | PI 610664 |