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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 702361 | 'HA-R20' | Helianthus annuus L. | North Dakota, United States | NC7 | | | 2023 | DEVELOPED | | | | | | | HA-R20 is a F3-derived F4 restorer selection from the cross of HA-DM2 and HA-R8. HA-DM2 (PI 687022) is a confection restorer line resistant to rust and DM released by USDA Sunflower and Biology Research Unit and the North Dakota Agricultural Experiment Station in 2017. HA-DM2 harbors the R12 rust gene and DM PlArg gene mapped to linkage groups (LGs) 11 and 1 of the sunflower genome, respectively. HA-R8 (PI 607511) is an oilseed restorer line resistant to rust released by USDA and the North Dakota Agricultural Experiment Station in 2001. The rust R gene in HA-R8 was named R15 and mapped to LG8. HA-R20 was developed by the pedigree breeding method and DNA marker-assisted selection for pyramiding of the rust R-genes R15 from HA-R8 and R12 from HA-DM2 and the DM R gene PlArg from HA-DM2. F1 hybrids were created by crossing HA-DM2 to HA-R8 in 2019. Homozygous pyramids with triple-gene combination, R12/R15/PlArg were selected using DNA markers from 188 F2 individuals and advanced to the F3 generation. The F3-derived HA-R20 were further evaluated for their reaction to rust and downy mildew infection and homozygosity for the both the rust R genes, R15 and R12, and DM R gene PlArg verified by DNA markers. HA-R20 is resistant to all known races of North American sunflower rust and all known races of the pathogen causing DM. Plant height of HA-R20 was 154 cm and flowered 64 days after planting in the field nursery at Glyndon, MN during the summer of 2021 | 2141561 | PI 702361 |
| 1 | PI 702362 | 'HA-R21' | Helianthus annuus L. | North Dakota, United States | NC7 | | | 2023 | DEVELOPED | | | | | | | HA-R21 is a F3-derived F4 maintainer selection from the cross of HA-DM3 and HA-R8. HA-DM3 (PI 687023) is a confection maintainer line resistant to rust and DM released by USDA Sunflower and Biology Research Unit and the North Dakota Agricultural Experiment Station in 2017. HA-DM3 carries the rust R gene R13a and DM R gene Pl17 mapped to sunflower LGs 13 and 4, respectively. HA-R8 (PI 607511) is an oilseed restorer line resistant to rust released by USDA and the North Dakota Agricultural Experiment Station in 2001. The rust R gene in HA-R8 was named R15 and mapped to LG8. HA-R21 was developed by the pedigree breeding method and DNA marker-assisted selection for pyramiding of the rust R-genes R15 from HA-R8 and R13a from HA-DM3 and the DM R gene Pl17 from HA-DM3. F1 hybrids were created by crossing HA-DM3 to HA-R8 in 2019. Homozygous pyramid with triple-gene combination, R13a/R15/Pl17 were selected using DNA markers from 188 F2 individuals and advanced to the F3 generation. The F3-derived HA-R21 were further evaluated for their reaction to rust and downy mildew infection and is homozygous for the both the rust R genes, R15 and R13a, and DM R gene Pl17 verified by DNA markers. HA-R21 is resistant to all known races of North American sunflower rust and all known races of the pathogen causing DM. Plant height of HA-R21 was 151 cm and flowered 68 days after planting in the field nursery at Glyndon, MN during the summer of 2021. ARS GIVES NO WARRANTIES OR GUARANTEES | 2141562 | PI 702362 |