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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 687022 | HA-DM2 | Helianthus annuus L. | North Dakota, United States | NC7 | | Not Available | 2018 | DEVELOPED | | | | | | Breeding material | HA-DM2 is a BC4F3-derived BC4F4 confection restorer selection from the cross of CONFSCL R5*5/RHA 464. CONFSCLR5 is a confection BC1F3-derived BC1F4 restorer selection from the cross of RO12-13//RHA 274/Dobritch/3/PSC 8/4/CONF/5/CONF released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2006. CONFSCLR5 is susceptible to downy mildew and rust infection. RHA 464 (PI 665015) is an oilseed restorer line released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2010 and is resistant to all North America downy mildew and rust races identified so far. The downy mildew and rust resistance genes in RHA 464 were named PlArg and R12 and mapped to linkage groups (LGs) 1 and 11 of the sunflower genome, respectively. HA-DM2 was developed by the backcross and pedigree breeding methods, with selection in each generation for downy mildew and rust resistance. In the BC4F2 generation, DNA markers linked to PlArg and R12, respectively, were used to select the homozygous BC4F2 individuals combining the two R-genes of PlArg and R12, with the selected homozygous double-resistant BC4F2 plants advanced to BC4F3 generation. Downy mildew and rust tests of the selected BC4F3 families in the greenhouse confirmed their homozygous nature. The homozygous BC4F3 seeds were grown in 16 rows of 20 plants at Glyndon, MN, in 2016, and the harvested BC4F4 seeds were bulked to form HA-DM2. | 1958035 | PI 687022 |
| 1 | PI 687023 | HA-DM3 | Helianthus annuus L. | North Dakota, United States | NC7 | | | 2018 | DEVELOPED | | | | | | Breeding material | HA-DM3 is a BC4F3-derived BC4F4 confection maintainer selection from the cross of HA-R6*5/HA 458. HA-R6 (PI 607509) is a confection maintainer line released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2001 and is resistant to all North America (NA) rust races identified so far. The rust resistance gene (R-gene) in HA-R6 was named R13a and mapped to LG13 of the sunflower genome. HA 458 (PI 655009) is an oilseed maintainer line released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2010 and is resistant to all NA downy mildew races identified so far. The downy mildew resistance gene in HA 458 was named Pl17 and mapped to LG4 of the sunflower genome. HA-DM3 was developed by the backcross and pedigree breeding methods, with selection in each generation for downy mildew resistance. In the BC4F2 generation, DNA markers linked to Pl17 and R13a, respectively, were used to select the homozygous BC4F2 individuals combining the two R-genes of Pl17 and R13a, with the selected homozygous double-resistant BC4F2 plants advanced to BC4F3 generation. Downy mildew and rust tests of the selected BC4F3 families in the greenhouse confirmed their homozygous nature. The homozygous BC4F3 seeds were grown in 16 rows of 20 plants at Glyndon, MN, in 2016, and the harvested BC4F4 seeds were bulked to form HA-DM3. | 1958036 | PI 687023 |
| 2 | PI 687024 | HA-DM4 | Helianthus annuus L. | North Dakota, United States | NC7 | | | 2018 | DEVELOPED | | | | | | Breeding material | HA-DM4 is a BC4F3-derived BC4F4 confection maintainer selection from the cross of HA-R6*5/HA-DM1. HA-R6 is HA-R6 (PI 607509) is a confection maintainer line released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2001 resistant to all North America (NA) rust races identified so far. The rust resistance gene (R-gene) in HA-R6 was named R13a and mapped to LG13 of the sunflower genome. HA-DM1 (PI 674793) is an oilseed maintainer line released by USDA-ARS and the North Dakota Agricultural Experiment Station in 2015 and is resistant to all NA downy mildew races identified so far. The downy mildew resistance gene in HA-DM1 was named Pl18 and mapped to LG2 of the sunflower genome. HA-DM4 was developed by the backcross and pedigree breeding methods, with selection in each generation for downy mildew resistance. In the BC4F2 generation, DNA markers linked to Pl18 and R13a, respectively, were used to select the homozygous BC4F2 individuals combining the two R-genes of Pl18 and R13a, with the selected homozygous double-resistant BC4F2 plants advanced to BC4F3 generation. Downy mildew and rust tests of the selected BC4F3 families in the greenhouse confirmed their homozygous nature. The homozygous BC4F3 seeds were grown in the greenhouse, and the harvested BC4F4 seeds were bulked to form HA-DM4. | 1958037 | PI 687024 |