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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 664478 | NM-10H | Cenchrus americanus (L.) Morrone | Nebraska, United States | S9 |  | | 2012 | DEVELOPED | | | | | | Genetic material | Genetic Type NM-lO is a genetic male sterile Fsline of pearl millet (Pennisetum glaucum (L) R. and Br.L Reg. no. GS-xx, PI xxxxxx, derived from a cross between a single male sterile plant found in line 34 -2B, and 68B. The male sterile plant was found in the Fs maintainer parent 34-2B in an isolation plot for the seed increase of cytoplasmic male sterile seed parent 34-2A4. The male sterility is maintained as a heterozygote (NM-10H) Parent line 34-2 resulted from multiple crosses involving experimental lines introduced from Serere Uganda, Zambia, Fort Hays Experimental Station KSU and IP 18293. 68B was selected from KS 79-2068B also from KSU. Genetic studies indicated that a single recessive nuclear gene, named mS3, is responsible for this male sterility. Anthers on mS3 plants are white, small (2-3mm long), thin, fully emerged but non-pendulous and without pollen. Female fertility is not affected. F1 plants grown from sibbed seed set on the original sterile plant (as a result of pollination from other plants in the maintainer line), were all normally male fertile. F2 populations were grown from four selfed F1 plants. Two F2's showed no male sterility, and two segregated for approximately 20% male sterile plants. Four different maintainer parents (23DBE(Burton,196S and 1981, Burton and Fortson,1966); NM-2B and NM-SB(Andrews et al. 2002), 68B(=843B),(Stegmeier et al. 1998), and two UNL R4 restorer parents (086A4R4 and 9RM/4RML were individually crossed to sterile plants. All F1 plants were male fertile. In the F2 populations of all crosses male sterile plants appeared and were sibbed. Planting sibbed seed from male sterile plants and sibbing to male-steriles again was continued for two more generations in three crosses. Three sibs were then grown out from each cross. The average occurrence of male sterile plants in three families from cross 34 -2B X 68B was 49% in 124 plants; 55% in 67 plants from cross 34-2B X 086A4R4 and 40% in 94 plants from cross 34-2B X 9RM/4RM. The phenotypic appearance of male sterility was similar over crosses and resembled that of the original male sterile source plant. The expression of mS3 male sterility was not affected by photoperiod ( less than 12hr daylength in winter greenhouse versus 14hrs days in field), or to variable temperatures. The seed stock for registration was made from sibbing male-steriles in the last generation from cross (34-2B X 68B). | 1897571 | PI 664478 |