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ACCESSIONPLANT NAMETAXONOMYORIGINGENEBANKIMAGEAVAILABILITYRECEIVEDSOURCE TYPESOURCE DATECOLLECTION SITECOORDINATESELEVATIONHABITATIMPROVEMENT LEVELNARRATIVE
0PI 692251Kronos EMS mutant T4-0865Triticum turgidum L. subsp. durum (Desf.) van Slageren California, United StatesNSGCKERNELS2020DEVELOPEDGenetic materialWe screened the Kronos EMS mutagenized population for mutations in the High Molecular Weight Glutenin Subunits (HMW-GS) Glu-B1x (subunit Bx6) using a CelI assay. We identified mutation C1198T (1st letter is the wild-type allele, last letter the mutant allele and the number the position from initial ATG) in line T4-0865. This mutation generates a premature stop codon (Q400*) in the encoded protein. Since the Bx6 subunit has 824 amino acids, the Q400* mutation is predicted to eliminate the N-terminal half of the protein. Since Kronos has non-functional mutant alleles for the A-genome Glu-A1x and Glu-A1y, the T4-0865 mutant only has the Glu-B1y functional HMW-GS. This was confirmed by SDS-PAGE. This germplasm is useful to test the effect of of the Glu-B1x subunit on pasta quality. [See PI 692251 - 692253 for related accessions.]2098638PI 692251
1PI 692252Kronos EMS mutant T4-1280Triticum turgidum L. subsp. durum (Desf.) van Slageren California, United StatesNSGCKERNELS2020DEVELOPEDGenetic materialWe screened the Kronos EMS mutagenized population for mutations in the High Molecular Weight Glutenin Subunits (HMW-GS) Glu-B1x (subunit Bx6) and Glu-B1y (subunit By8) using a CelI assay. We found mutant line T4-1280 with truncation mutations in both Glu-B1x and Glu-B1y. The C349T mutation in Glu-B1x (1st letter is wild-type allele, last letter mutant allele and number the position from initial ATG) results in a premature stop codon (Q117*) that eliminates 86 % of the N-Terminal part of the protein. The C190T mutation in Glu-B1y resulted in an early stop codon (R64*) that eliminated 91% of the protein. Since Kronos has non-functional mutant alleles for the A-genome Glu-A1x and Glu-A1y, the T4-1280 mutants has no HMW-GS. This was confirmed by SDS-PAGE. This germplasm is useful to test the effect of introduced natural or synthetic HMW-GS on pasta quality or alergenicity. [See PI 692251 - 692253 for related accessions.]2098639PI 692252
2PI 692253Kronos EMS mutant T4-2197Triticum turgidum L. subsp. durum (Desf.) van Slageren California, United StatesNSGCKERNELS2020DEVELOPEDGenetic materialWe screened the Kronos EMS mutagenized population for mutations in the High Molecular Weight Glutenin Subunits (HMW-GS) Glu-B1y (subunit By8) using a CelI assay. We identified mutation C1354T (1st letter is the wild-type allele, last letter the mutant allele and the number the position from initial ATG) in line T4-2197. This mutation is predicted to generate a premature stop codon (Q451*) that eliminates 37% of the N-terminal part of the By8 protein (wild-type protein has 720 amino acids). Since Kronos has non-functional mutant alleles for the A-genome Glu-A1x and Glu-A1y, the T4-2197 mutant only has the Glu-B1x functional HMW-GS. This was confirmed by SDS-PAGE. This germplasm is useful to test the effect of the Glu-B1y gene on pasta quality or alergenicity. [See PI 692251 - 692253 for related accessions.]2098640PI 692253