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| ACCESSION | PLANT NAME | TAXONOMY | ORIGIN | GENEBANK | IMAGE | AVAILABILITY | RECEIVED | SOURCE TYPE | SOURCE DATE | COLLECTION SITE | COORDINATES | ELEVATION | HABITAT | IMPROVEMENT LEVEL | NARRATIVE | | |
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| 0 | PI 701905 | Kronos-PS | Triticum turgidum L. subsp. durum (Desf.) van Slageren | California, United States | NSGC |  | | 2023 | DEVELOPED | | | | | | Genetic material | Kronos is a spring wheat that carries a functional Vrn-A1 allele for spring growth habit (Vrn-A1c allele), a functional Vrn-B2 long day repressor locus (genes ZCCT-B2a and ZCCT-B2b), and the Ppd-A1a allele for reduced photoperiodic response. We developed a near-isogenic Kronos line carrying the wild-type photoperiod-sensitive Ppd-A1b allele (Kronos-PS), which flowers very late under short day and approximately five days later than the photoperiod insensitive Kronos under long days (Pearce et al. 2017). This line was developed by crossing Kronos with the tetraploid durum variety Langdon (which carries the Ppd-A1b allele) followed by three backcrosses to Kronos as the recurrent parent, self-pollination, and selection of homozygous plants with molecular markers. The seeds provided here are BC3F4 seeds homozygous for the Ppd-A1b allele and were used in additional studies where they were combined with mutations in co1 and co2 (Shaw et al. 2020), and with mutation sin elf3 and phyB (Alvarez et al. 2022). | 2141272 | PI 701905 |
| 1 | PI 701907 | Kronos elf3 ppd1 knock-out | Triticum turgidum L. subsp. durum (Desf.) van Slageren | California, United States | NSGC |  | | 2023 | DEVELOPED | | | | | | Genetic material | Kronos is a spring wheat that carries a functional Vrn-A1 allele for spring growth habit (Vrn-A1c allele), a functional Vrn-B2 long day repressor locus (genes ZCCT-B2a and ZCCT-B2b), and the Ppd-A1a allele for reduced photoperiodic response. For the elf3 loss-of-function mutant, we combined the A-genome mutation W532* found in Kronos-982 with the B-genome mutation Q526* found in Kronos-3669. These premature stop codons eliminate the last 241 (A-genome) and 244 (B-genome) amino acids of the C-terminal region, including the two conserved blocks of the ELF3 protein (Alvarez et al., 2016).
For the ppd1 loss-of-function mutant, we combined the A-genome mutation W154* found in Kronos-689 (that eliminates 514 of the 668 amino acids including the conserved CCT domain) with a B-genome gamma-ray induced deletion identified in the hexaploid wheat Paragon and transferred into Kronos by three backcrosses (which eliminates all PPD-B1 gene copies) (Pearce et al. 2017).
The resulting BC2F3 loss-of-function elf3 and ppd1 lines were intercrossed and lines homozygous for the four mutations (elf-A3 elf-B3 ppd-A1 ppd-B1) were selected with molecular markers and are the material deposited here. These plants head in ~70 days from sowing.
| 2141274 | PI 701907 |