Evaluation location: New York, United States
2024 Germplasm Evaluation of diverse hemp germplasm in an outdoor field in Geneva NY for Septoria leaf spot and powdery mildew, see USDA-ARS PN 8060-21000-034-031-A [https://www.ars.usda.gov/research/project/?accnNo=445656]. Trial run as a collaboration between Cornell University (Larry and Chris Smart laboratories) and USDA-PGRU Hemp Germplasm Laboratory in McCarthy 01. Pathogen data and associated information generated by Jocelyn Schwartz (lead author), Chris Smart, Larry Smart, Tyler Gordon, and Zachary Stansell and others [Add additional names given by JAS]. JAS verbatim: “Seeds were planted in flats on 15 May, where they were regularly maintained and watered. The transplants were set in the field by hand into 4 ft wide raised beds covered with plastic mulch on 30 May. Each plot consisted of 5 plants with 3 ft spacing in between plants, 3 ft spacing in between plots, and 7.5 ft spacing in between rows. The field was established in a randomized complete block design with 3 repetitions of 105 accessions. Plots were irrigated and fertigated as needed via a drip line with 24-8-16 Miracle Gro all-purpose, water-soluble fertilizer. Plastic weed cloth was applied in between rows and was supplemented with hand-weeding as needed. Inoculum was created with a single conidial isolate of Septoria cannabis collected from Geneva, NY. 100 μl/L of Silwet was added to the inoculum and a final concentration of 6.4 *10^5 conidial suspension was applied using a battery-powered backpack sprayer on 3 July. Approximately 30 mL of inoculum was applied to each plant. Symptoms were first observed 7 days post inoculation. Disease severity ratings were visually estimated on a scale of 0 to 100%. The first disease severity rating was on 10 July, and disease severity continued to be visually estimated each week.