Evaluation location: , China
In our previous screening for resistance to late blight using a detached leaf assay, we identified the diploid wild type JAM1-4 (S. jamesii), which originated from the United States, was highly resistant to the super race strain 2013-18-306. In this study, we used potato tissue culture plants for live inoculation with P. infestans. The tissue culture plants of JAM1-4 (S. jamesii) were cultured in 30 ml of MS medium and grown for 4 weeks under the conditions with 16 h of light, 8 h of dark, light intensity of 2,000 lx, and temperature of 22°C. Then the 8 to 10 tissue culture plants of each bottle were inoculated with P. infestans or as control.