Evaluation location: Oregon, United States
A 25 KASP SNP genotyping assay for Humulus identification. Tissue for KASP genotyping were shipped after the addition of silica to each well using the Plant Sample Collection kit (according to the manufacturer’s recommendations) to LGC Biosearch Technologies, where DNA extraction and genotyping was conducted. Raw allele calls for the 25 assays were received from LGC Biosearch Technologies and assessed via their SNPviewer software.