Evaluation location: Puerto Rico, United States
One hundred fifty-seven accessions from the NPGS Senegal collection were evaluated for anthracnose resistant response at Isabela, Puerto Rico in 2014 and at Mayaguez, Puerto Rico in 2015. The experimental design at both locations was a randomize block design of two blocks. The disease assessments method was based on a scale 1 - 5, where 1 = no symptoms or chlorotic flecks on leaves; 2 = hypersensitive reaction on inoculated leaves, but no acervuli in the center; 3 = infected bottom leaves with acervuli; 4 = necrotic lesions with acervuli on bottom leaves showing spreading to middle leaves; and 5 = most leaves dead because of infection, and flag leaf infected (Prom et al. 2009). This rating system was then categorized into resistant (rated as 1 and 2) and susceptible (rated as 3–5) reaction classes.