Evaluation location: Illinois, United States
Total oil content was determined by Pulsed Nuclear Magnetic Resonance (pNMR) on a Bruker mq-CU 20-series, Firmware v 2.50Rev.00 using factory instrument setting 909_18A, NMR frequency 19.98 MHz at 40C and pNMR instrument settings of: digital bandwidth 20,000kHZ; gain 63dB; 16 scans with 2 sec recycle delay; pulse separation 3.5ms with sampling window of 6.9583 - 7.0583ms. Seed samples weighed to the tenth mg were placed into Pyrex No. 9820 vials and heated to 40C for 2 hr before analysis. Response factors for each sample were measured and reported to the tenth mg of oil based on standard curves developed using canola oil.