METHOD: Glycoalkaloid assay CONDITIONS: Green House - 16h light at 27C/20C day/night METHOD NARR: Glycoalkyloids were separated by HPTLC. Co-chromatography with standards and reverse phase HPLC were used to confirm the identificevelopment times varied to distinguish extracts present in high vs. low concentrations. RESULTS NARR: The new technique being tested was not reliable in detecting solanine or leptinine II. Leptine I was readily detected.458313 contained 420mg/100g of leptine I + II while chc 320287 contained 160mg/100g of the leptines. AUTHOR EXPLANATION: Field and laboratory tests show that leptine levels below 20mg% only weakly deter CPB, or not at all. Tce for setting the detection level at 20mg% or above. Using the TLC method, 80 samples per day can be processed compared to 10 for GLC and 4 for HPLC.