METHOD: Ion Leakage CONDITIONS: Green House - 18h light at 15-20C METHOD NARR: Plants were cold acclimated in a growth chamber either at 2-4C or 1-2C in a 14h photoperiod. High and low light intensities were used. Temperature w 2C per hour from 10C to either non-freezing (2C) or freezing (-3C) night frost. Molecular tests were used to examine induction of the Scgst1 gene, which codes for glutathione S-transferase, and it's activity levels. RESULTture reglated GST was isolated from cmm. A single copy of the gene (Scgst 1) was found in both cmm and tbr. Accumulation of the transcript was seen in cmm but not tbr under excess light/low temperature conditions. Cmm x tbr h levels of accumulation. The association between frezing tolerance and Scgst 1 transcript was lost in S1 genotypes. AUTHOR EXPLANATION: Adaptation to low temperature is based on numerous mechanisms.