Evaluation location: New York, United States
2023 Germplasm Evaluation for septoria and powdery mildew. Trial run by USDA-PGRU and Cornell University in McCarty 06. Data generated by Jocelyn Schwartz, Chris Smart, Dan Meyers, Tyler Gordon, and Zachary Stansell. [JAS verbatium: “Seeds were planted in flats on 11 May, where they were regularly maintained and watered. Prior to transplanting in the field, Big Yield Blended 19:6:19 Fertilizer was broadcast at a rate of 500 lbs/acre. The transplants were set in the field by hand into 4 ft wide raised beds covered with plastic mulch on 31 May. Each plot consisted of 5 plants with 3 ft spacing in between plants, 3 ft spacing in between plots, and 7.5 ft spacing in between rows. The field was established in a randomized complete block design with 3 repetitions of 81 accessions. Plots were irrigated and fertigated as needed via a drip line with 24-8-16 Miracle Gro all-purpose, water-soluble fertilizer. Plastic weed cloth was applied in between rows and was supplemented with hand-weeding as needed. Inoculum was created with a single conidial isolate of Septoria cannabis collected from Geneva, NY. 100 μl/L of Silwet was added to the inoculum and a final concentration of 4.9 *10^4 conidial suspension was applied using a battery-powered backpack sprayer on 22 June. Approximately 30 mL of inoculum was applied to each plant. Symptoms were first observed 7 days post inoculation. Disease severity ratings were visually estimated on a scale of 0 to 100%. The first disease severity rating was on 29 June, and disease severity continued to be visually estimated each week.”]