Evaluation location: Puerto Rico, United States
A total of 756 and 760 accessions were evaluated for sugar content (i.e. Brix) in 2015 and 2016, respectively. Each year these accessions were grown in a completely randomized block design with plots measuring 1.8 m in length with 0.9 m between rows. When the plot reached its physiological maturity (30-45 days after flowering), three to five plants per plot (leaf and stalk) were pressed with a three-roller sugarcane mill (Raja-1 US Ice Machine Manufacturing Co. FL, US) to collect the total juice. The Brix of the total juice was measured using a handle refractometer (Atago U.S.A. Inc., Bellevue, WA).