Evaluation location: Puerto Rico, United States
Sixty-eight accessions from the NPGS Zimbabwe collection were evaluated for anthracnose resistant response in 2010 and 2011. The experimental design for both year was a randomize block design of three blocks. The disease assessments method was based on a scale 1 - 5, where 1 = no symptoms or chlorotic flecks on leaves; 2 = hypersensitive reaction on inoculated leaves, but no acervuli in the center; 3 = infected bottom leaves with acervuli; 4 = necrotic lesions with acervuli on bottom leaves showing spreading to middle leaves; and 5 = most leaves dead because of infection, and flag leaf infected (Prom et al. 2009). This rating system was then categorized into resistant (rated as 1 and 2) and susceptible (rated as 3–5) reaction classes.