Evaluation location: Puerto Rico, United States
Two hundred fifty-five accessions from the NPGS Sudan core collection were evaluated for anthracnose resistant response at Isabela and Mayaguez, Puerto Rico in 2014 and 2016, respectively. At both locations, the experimental design was a complete randomize block with plots measuring 1.8 m length. Sixty-six accessions that were resistant at both locations were evaluated for one additional year (2017) at Isabela, Puerto Rico in randomized complete block design consisting of three blocks with plots of 1.8 m length. The disease assessments method was based on a scale 1 - 5, where 1 = no symptoms or chlorotic flecks on leaves; 2 = hypersensitive reaction on inoculated leaves, but no acervuli in the center; 3 = infected bottom leaves with acervuli; 4 = necrotic lesions with acervuli on bottom leaves showing spreading to middle leaves; and 5 = most leaves dead because of infection, and flag leaf infected (Prom et al. 2009). This rating system was then categorized into resistant (rated as 1 and 2) and susceptible (rated as 3–5) reaction classes.