Evaluation location: Puerto Rico, United States
Three hundred twenty-two accessions from the NPGS Ethiopian core collection were evaluated for anthracnose resistant response at Isabela and Mayaguez, Puerto Rico in 2013 and 2014, respectively. At both locations, the experimental design was a complete randomize block with plots measuring 1.8 m length. One hundred fifty-two accessions that were resistant at both locations were evaluated for two additional years (2015 and 2018) at Isabela, Puerto Rico in randomized complete block design consisting of two blocks with plots of 1.8 m length. The disease assessments method was based on a scale 1 - 5, where 1 = no symptoms or chlorotic flecks on leaves; 2 = hypersensitive reaction on inoculated leaves, but no acervuli in the center; 3 = infected bottom leaves with acervuli; 4 = necrotic lesions with acervuli on bottom leaves showing spreading to middle leaves; and 5 = most leaves dead because of infection, and flag leaf infected (Prom et al. 2009). This rating system was then categorized into resistant (rated as 1 and 2) and susceptible (rated as 3–5) reaction classes.