Evaluation location: , United States
Three hundred thirty-five accessions from the sorghum association panel were evaluated for anthracnose resistant response at Isabela and Mayaguez, Puerto Rico in 2013 and 2014, and at Tifton, Georgia in 2017. At the three locations, the experimental design was a complete randomize block, with plots measuring 1.8 m and 4.0 m in length at Puerto Rico and Georgia, respectively. A subset of 46 resistance accessions were evaluated for anthracnose resistance at Isabela, Puerto Rico in 2015 and 2016, using a randomize complete block design consisting of three blocks. The disease assessments method was based on a scale 1 - 5, where 1 = no symptoms or chlorotic flecks on leaves; 2 = hypersensitive reaction on inoculated leaves, but no acervuli in the center; 3 = infected bottom leaves with acervuli; 4 = necrotic lesions with acervuli on bottom leaves showing spreading to middle leaves; and 5 = most leaves dead because of infection, and flag leaf infected (Prom et al. 2009). This rating system was then categorized into resistant (rated as 1 and 2) and susceptible (rated as 3–5) reaction classes.