Evaluation location: Puerto Rico, United States
In an effort to characterize protein content in depth, a large collection of bamboo germplasm accession leaf samples was assembled and analyzed. A total of 231 leaf samples were collected for both tropical and temperate bamboo species from public (USDA-ARS) and private collections in Mayaguez, Puerto Rico and Griffin, Georgia. Samples were analyzed for protein content by estimating total nitrogen using a modification of the micro-Kjeldahl method.