Evaluation location: Washington, United States
25 ng genomic DNA was used as template for SNP genotyping carried out using a MALDI-TOF mass spectrometer (Sequenom). SNP assays were designed from previously published SNPs (Deulvot et al. 2010, BMC Genomics 11:486) using software iPLEX GOLD Assay (Sequenom). The data was analyzed with MassArray analyzer software (Sequenom) which called the biallelic markers. Each SNP was scored for each sample A, T, G or C.
An Excel file with the markers and genotypes