Evaluation location: Madrid, Comunidad de, Spain
Medicgo truncatula seeds were scarified with sandpaper and sterilized in 50% (v/v) commercial bleach for 15 min, then they were rinsed (4?20 min) with sterile water. Seeds were imbibed in sterile water at 4 degrees C overnight and then germinated on 1% agar/water in Petri dishes in growth chamber conditions (25/19 degrees C, 16/8 h) for 48 h in darkness. Seedlings were transferred to the so-called Fahraeus slide hydroponic system, as described previously (Heidstra et al., 1994; Ortega-Villasante et al., 2005) with some modifications. A modified Hoagland nutrient solution was used. The relative root growth (RRG) of the seedlings was used as an indicator of metal tolerance, measuring the root length of the seedlings before and after treatment, and expressing the increase in length relative to that of control seedlings grown in the absence of Hg.
See the publication: 'Environmental and Experimental Botany 91 (2013) 90-96' for more detailed information on how the experiment was preformed.
Note many of the National Plant Germplams System (NPGS) accessions used in this experiment now have 'PI' numbers instead of 'W6' numbers. See the comment column in the GRIN data to find the W6 number that corresponds to the PI number.
For a pdf file of the article.
For a pdf file of the data.