Evaluation location: Washington, United States
Seeds of 32 Brachypodium distachyon accessions were started in germination boxes on March 3, 2010 and transplanted to flats when they germinated and were large enough to handle. They were grown in the greenhouse for three weeks, then transferred to a vernalization chamber on a 12 hour daylength with metal halide lightning for six weeks. After hardening off in a lathhouse, they were planted in the field in Pullman, Washington on June 1. There were four replications in a randomized block design. Each plot consisted of 5 plants 1 foot apart in a single row. There was a 5 foot alley between each plot. The plants were watered twice for establishment.
Head numbers were converted to a 1-5 coded scale via this ratio: 1 = 1-10 heads, 2 = 11-20 heads, 3 = 21-60 heads, 4 = 61-80 heads, 5 = 81-100 heads.