Evaluation location: Illinois, United States
Calendula accessions held at the USDA-ARS North Central Regional Plant Introduction Station (NCRPIS) located in Ames, IA were shipped to the USDA-ARS National Center for Agricultural Utilization Research Station (Bio-Oils Research Unit) located in Peoria, IL to be analyzed for their total-oil and fatty-acid methyl-ester (FAME) profiles. Approximately 100 seeds from each accession were shredded in a low-volume coffee mill before analysis in a Bruker MQ 20 pulsed Nuclear Magnetic Resonance (pNMR) instrument and determination of moisture by the American Oil Chemists? Society (AOCS) method Ca 2c-25 for calculation of total oil on a dry-weight basis. FAME profiles were prepared in triplicate by using 0.3 gram samples reacted with 0.25M sodium methoxide at 65C for 30 minutes before extraction with hexane and a saturated sodium chloride wash, and then analyzed by a Hewlett Packard 5890 gas chromatograph. Please note that some concentrations for palmitolenic acid were not measured and are presented as zero, as those values were below detection levels of the intstrumentation used.