Evaluation location: Georgia, United States
Evaluation for root rot resistance (
Phytophthora capsici) in peppers.
Three virulent isolates each from the A1 and A2 mating types of Phytophthora capsici were used in the mass screening and subsequent inoculation tests of peppers. A mixture of zoospores from these isolates were used in inoculating the test plants. Seeds from each accession were sown in plastic cells of a multipot bedding plant container. Two hours prior to inoculation, trays containing 14-day-old pepper seedlings were placed in water-filled trays to saturate the roots. A five ml zoospore suspension was then delivered to each cell using an automatic pipette dispenser resulting in a concentration of 10,000 zoospores per cell. Disease evaluation was performed 14 days after inoculation. The plants were evaluated based on a 10-point scale with even numbers corresponding to intermediate response. A disease index value of 2 or less was considered resistant, and a value greater than 2 was susceptible.
View more detailed information on Materials and Methods (.pdf file).