BARLEY.BETAGLUCAN.ABERDEEN.08

 
Evaluation location: Idaho, United States
Laboratory experiments, Aberdeen, Idaho. Data recorded for beta-glucan, protein, and lipid contents. Moisture was determined according to an official method (AOAC 2002) and was used to convert the contents of other components into a dry matter basis. The total nitrogen/protein content was measured by a combustion method (AOAC 2002), using a protein analyzer (Model FP-528, Leco Corp. St. Joseph, MI). The protein content was calculated with a conversion factor of 5.75. The oil (lipid) content was determined by an AOCS Official procedure (AOCS 2005), using a fat analyzer (Model XT 10, Ankom Technology, Macedon, NY). However, instead of using petroleum ether, hexane was used as the extracting solvent. Beta-glucan determinations were based on the method of Hu and Burton, 2008.
Citation(s)
  • Hu, Gongshe & C. Burton. 2008. Modification of the standard enzymatic protocol to a cost-efficient format for mixed-linkage (1->3, 1->4) beta-D-glucan measurement. J. Agric. Food Chem. 85:648-653.