POTATO.RINGROT.ISHIMARU.1994

 
METHOD: Both IFAS and Visual Examination CONDITIONS: Green House - minimum of 12h light. Temperature ranged from 20-32C. METHOD NARR: Tissue-cultured plantlets were air dried 20 minutes then their roots were immersed in 10ml ofor 20 minutes. They were then planted in soil and monitored for ring rot for 72 days. IFAS was used to detect bacterial cells in stem sections. RFLP and RAPD analyses were also performed. RESULTS NARR: Two accessions weterial ring rot. The results identify parents suitable for future mapping of genes for immunity or resistance. AUTHOR EXPLANATION: The number of cells per well was recorded as the average # of cells viewed in 10 fields. IFA1=0-10 cells/field, 2=11-20 cells/field, 3=21-50 cells/field, 4=>50 cells/field. Bacterial ring rot reactions were based on a combination of IFAS and incidence of symptomatic plants: Susceptible= >15% symptomatic plants and IFAS of 1-symptomatic plants and IFAS of 1-4. Highly Resistant= 0% symptomatic plants and IFAS of 0.1-0.9. Immune= 0% symptomatic plants and IFAS of 0.
Trait(s) evaluated
Citation(s)
  • Ishimaru, C. A., N. L. V. Lapitan, A. VanBuren, A. Fenwick, & K. Pedas. 1994. Identification of parents suitable for molecular mapping of immunity and resistance genes in Solanum species. Amer. Potato J. 71:517-533.