METHOD: ELISA, ISEM and Visual Examination CONDITIONS: Green House - 18h light with light intensity 150umol. Daily minimum temp 19C, maximum temp 24C. METHOD NARR: In vitro plantlets were transplanted to soil. The viruses wereplants of each clone, in each of the 2 or 3 experiments carried out for each strain, both mechanically and by graft. ELISA and ISEM were used for detection 21 and 28 days after mechanical and graft inoculation respectively.ed for PVA. AUTHOR EXPLANATION: Differences in response to inoculation by mechanical means versus grafting are noted. Temperature can affect the development of necrosis with HR rendered less effective at higher temps. Remarstance phenotyoes were determined.