METHOD: Glycoalkaloid Assay CONDITIONS: Field METHOD NARR: Glycoalkaloid content was determined using the column and HPLC analytical method of Sinden et al 1986. Additionally, high-performance thin-layer chromatography was used RESULTS NARR: The average weight of the 1992 chc sample tubers were significantly lower than in all other populations. The F2 and F4 population tubers were significantly smaller than those of the tbr parent. In 1992 chcne content compared to tbr. Mean sol contents in F2 and F4 pops. were significantly lower than chc but significantly higher than tbr. The mean chac:sol ratios for chc were lower than other populations. TGA of chc consisted one. AUTHOR EXPLANATION: This is a companion paper to Sanford et al 1994 and the same plants were used for both papers. When comparing the the foliage and tuber results obtained, the correlation coefficients were not significant. Thmplications of the fact that tuber size plays a role in the results obtained.