METHOD: Glycoalkaloid Assay CONDITIONS: Green House - 16h light METHOD NARR: Quantification of glycoalkaloids was performed by analytical HPLC with a-solanine as the chromatographic standard. TLC was used to futher identify thosed from known glycoalkaloids. To evaluate the effect of altitude, accessions were divided into discrete elevational classes and statistically analyzed. RESULTS NARR: There was a strong correlation between and within leptie also positive correlations between solanine and chaconine, which were found in all accessions tested. A significant trend was observed between TGA and elevation, with TGA decreasing with increasing elevation. Concentrations, chaconine and TGA, but not of leptines or leptinines were negatively correlated with altitude. AUTHOR EXPLANATION: The inheritance of genes for leptine synthesis is complex, having quantitative aspects and possibly involving envirc interactions. Like solanine and chaconine, leptinine only weekly deters feeding by CPB. The likelihood that solanine and chaconine function as allelochemicals, in planta, is rather high.