METHOD: Leptine Assay CONDITIONS: Green House - 16h light METHOD NARR: F1, interF1 and backcross families were assayed forTGA and foliar leptine production. Crosses were made between high (>62%) and low (<17%) producers of lepties. Specific glycoalkaloids were quantified by analytical HPLC. RESULTS NARR: Based on F1 segregation, it appears that high leptine proportion is controlled by a recessive gene. The presence of leptine in all hybrids ofl effect of the tubersoum parent on the amounts and proportions of leptines in the hybrids, indicates polygenic control by dominant gene(s). Unexpected segregation ratios were found. The F1 generation analysis indicates homozye the interF1 and backcross generations indicate a more complex inheritance model which involves a second major gene. AUTHOR EXPLANATION: Light is known to affect glycoalkaloid levels so all plants recieved supplemental light to 16h