METHOD: Glycoalkaloid Assay CONDITIONS: Green House and Tissue Culture METHOD NARR: A new procedure was developed that combines the extraction and hydrolysis of glykoalkaloids into a single step and uses capillary GC to quantita glykoalkaloid (SGA) aglycons from leaves and tubers. This process avoids ammonia precipitation and uses tomatine as an internal standard. RESULTS NARR: Chc leaves contained higher levels of alkaloids than tubers. In GHnidine 54%, leptinidine 36% and solanidine 10%. The trend differed in plant grown in vitro with solanidine 66%, acetylleptinidine 20% and leptinidine 14%. Acetylleptinidine was not detected in chc tubers. AUTHOR EXPLANATION:uantitatively influenced by the environment. The choice of tomatine as an internal standard was suitable for this experiment because, in the case of chc, it meets the 5 major criteria used to define a good internal standard. In futurrd must be determined by considering which glykoalkaloids are likely to be found for the species being investigated.