METHOD: Ion Leakage CONDITIONS: Green House - 14h light at 14-18C METHOD NARR: Leaflet and callus tissue were evaluated for frost tolerance. Freezing was achieved by both submersion and non-submersion techniques. Frost damage oby electrolyte leakage conductivity. The temperature resulting in 50% viability reduction was recorded. Comparison of frost tolerance with other biochemical factors (Amino acids, Proline and Electrolytes) was also determinerost tolerance in acl is associated with AA contents and electrolyte levels, and to a lesser extent Proline levels, indicating additional frost tolerance mechanisms may be involved. Acl is the only species that showed sig. higr in both callus and cell suspensions. Mga & cmm were more tolerant to frost at the plant level, while plt was more tolerant as callus tissue. AUTHOR EXPLANATION: Submerged freezing causes more damage to cells than non-submerged f the frost tolerance measures. Frost tolerance of some wild Solanum species is poorly refelcted in frost-killing temperature of cell culture, whether as callus or as suspensions.