METHOD: Salt Stress CONDITIONS: Growth Chamber - 14h light at 18-22C or 2-4C METHOD NARR: NaCl solution was added to the culture medium of five-week-old plantlets. Three groups were grown under varying conditions: temperate fors, temperate for 7 days with ABA added. Cold hardiness was measured by an electrical conductivity test. The freezing temperature that caused a 50% loss of total leachate was designated as the killing temperature. Abscisicrified and immunoassay was performed. After 1-day of salt treatment, and 3 days of cold- and ABA-treatments, 2 leaflets per sample were radioactively labeled on the abaxial surface of the leaflets. Proteins were extracted and q ABA treatment increased the cold hardines by about 6C by the fifth day. Salt treatment in liquid culture medium resulted in a 3C increase in cold hardiness which is less than both the ABA- and cold-treatments. The development of colstress occurred immediately after the transitory increase in free ABA. Salt treatment resulted in alterations of protein synthesis pattern in the leaf tissue. AUTHOR EXPLANATION: Results suggest that the salt-induced sive proteins is associated with the induction of cold hardiness of the plantlets by salt stress. Results also suggest that these 3 proteins may play a major role in the acquisition of cold hardiness in response to co