METHOD: Ion Leakage CONDITIONS: Biotron - 14h light at 18-20C or 2-4C METHOD NARR: Freezing injury was measured by ion leakage in acclimated and unacclimated samples. A glycol bath was used to chill leaf samples by lowering the For chloroplast analysis total DNA was isolated, digested, and blotted onto a membrane. Petunia probes were used to distinguish the chloroplast contribution of tbr and cmm in progeny. RESULTS NARR: All species showed ane with acclimation. In somatic hybrids between tbr and brd, little of the acclimation ability from brd was expressed. In somatic hybrids between tbr and cmm the acclimation ability was intermediate to the two parents. AUTHORybrids, the sensitive-to-hardy genomic ratio could be an important determinant in the expression of acclimation vs. nonacclimation.