Evaluation location: , Japan
National Plant Germplasm System Pisum accessions were screened by mechanical inoculation with CIYVV-Pst1/CP(15) and symptom development was observed for about 2 weeks. Lines that showed no symptoms were selected, and further inoculation tests were conducted. These lines were then screened for systemic infection by mechanical inoculation using indirect ELISA and green fluorescent protein (GFP)-tagged ClYVV detection.