Evaluation location: Idaho, United States
Sugar beet seeds were planted in the greenhouse in 500 cm3 pots containing naturally infested beet cyst nematode soil (4.3 eggs and larvae per 1 cm3 soil). PI accessions were compared to the susceptible check, HM WSPM9 (OB=9). Experimental design was randomized block with six replications (pots of 5 seedlings each). Sugar beet seedlings were separated from soil ten weeks after planting. Beet cyst nematode females and cysts were enumerated from the roots and soil. An analysis of variance was performed on the data, and mean separation was computed using the least significant difference. A numeric score of 0 to 9 was assigned to each PI accession (0 = immune, 9 = highly susceptible).